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PMID: 6549112 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Immunohistochemical localization of cardiodilatin in myoendocrine cells of the cardiac atria.

Anatomy and embryology ·Vol. 170 ·No. 2 ·1984-00-00 ·Pages 123-7

Metz J, Mutt V, Forssmann WG

Abstract

Region-specific antibodies against synthetic N-terminal fragments of cardiodilatin (CDD) were raised in rabbits and used for the immunohistochemical detection of this new peptide hormone in the myoendocrine cells within the cardiac atria of several species. The peroxidase-antiperoxidase (PAP) and fluorescein isothiocyanate (FITC) immunohistochemical methods gave identical results of cardiodilatin-immunoreactivity (CDD-IR) within the tissue. In addition to the porcine right atrial appendage, myoendocrine cells with CDD-IR were also detected in the left atrium of porcine heart, as well as in other species such as dog and cat. The exact localization of the immunoreactivity in specific secretory granules was mostly related to the Golgi-area which is located on both nuclear poles of auricular myoendocrine cells. The results confirm that cardiodilatin is stored in secretory granules observed through electron microscopical means. This hormone is most likely synthesized and released in myoendocrine cells, exerting its important cardiovascular effects.

MeSH Terms
Animals Atrial Natriuretic Factor Cats Dogs Endocrine Glands/analysis,cytology Heart Atria/metabolism Histocytochemistry Immunochemistry Muscle Proteins/analysis Muscles/cytology,metabolism Myocardium/analysis Swine Tissue Distribution
Chemicals
Muscle Proteins Atrial Natriuretic Factor cardiodilatin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Metz J
Mutt V
Forssmann W G
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29 references, click to expand
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Article Info
Journal
Anatomy and embryology
Abbr.
Anat Embryol (Berl)
ISSN
0340-2061
DOI
10.1007/BF00318996
Published
1984-00-00
Pages
123-7
Language
English
Region
Germany
NLM ID
7505194
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00318996
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