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PMID: 6557026 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Nuclear matrix and hnRNP share a common structural constituent associated with premessenger RNA.

The EMBO journal ·Vol. 2 ·No. 6 ·1983-00-00 ·Pages 953-60

Gallinaro H, Puvion E, Kister L, Jacob M

Abstract

Nuclear matrix and heterogeneous nuclear ribonucleoprotein (hnRNP) were compared to establish whether premessenger RNA (premRNA) was associated with a same constituent in both structures. The isolation of nuclear matrix included the removal of chromatin and of 0.4 M KCl-soluble material. HnRNP, isolated by a standard method was also treated by 0.4 M KCl. Both isolation procedures caused the removal of DNA, histones, a fraction of small nuclear RNA and of nonhistone proteins including the hnRNP proteins in the 30 000-40 000 mol. wt. range. High resolution autoradiography showed that hnRNA remained associated with the residual fibrils in both structures. They both contained the same premRNA and maturation products as shown by the analysis of the transcripts of the early region 3 of adenovirus 2. In addition, the small nuclear RNA and protein of the salt-resistant complexes were also present in the matrix. The results are compatible with the idea that the salt-resistant complexes from hnRNP constitute the fibrils associated with premRNA in the nucleoplasmic matrix. The fibrils may be the basic unit of splicing and their organization in matrix might provide the spatial configuration necessary for regulation.

MeSH Terms
Cell Nucleus/metabolism,ultrastructure HeLa Cells/metabolism Heterogeneous-Nuclear Ribonucleoproteins Humans Microscopy, Electron Nucleic Acid Precursors/metabolism Potassium Chloride RNA Precursors RNA, Messenger/metabolism Ribonucleoproteins/metabolism
Chemicals
Heterogeneous-Nuclear Ribonucleoproteins Nucleic Acid Precursors RNA Precursors RNA, Messenger Ribonucleoproteins Potassium Chloride
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Gallinaro H
Puvion E
Kister L
Jacob M
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41 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1983-00-00
Pages
953-60
Language
English
Region
England
NLM ID
8208664
PMCID
PMC555214
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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