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PMID: 656066 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Glycosyl transfer from nucleotide sugars to C85- and C55-polyprenyl and retinyl phosphates by microsomal subfractions and Golgi membranes of rat liver.

The Biochemical journal ·Vol. 172 ·No. 1 ·1978-04-15 ·Pages 123-7

Bergman A, Mankowski T, Chojnacki T, De Luca LM, Peterson E, Dallner G

Abstract

The capacity of isolated membrane fractions to catalyse transfer of sugars from sugar nucleotides to alpha-saturated and non-saturated forms of phosphorylated C85 and C55 polyprenols and retinyl phosphate was examined. The amount of endogenous lipid acceptor present for various sugars was also measured. It appears that the types and amounts of polyprenyl phosphates present in rough- and smooth-microsomal fractions and Golgi membranes are different and the individual polyprenyl phosphates exhibit specificity as sugar acceptors.

MeSH Terms

Animals Cell Membrane/metabolism Diterpenes Golgi Apparatus/metabolism In Vitro Techniques Liver/metabolism Male Membrane Lipids/metabolism Membrane Proteins/metabolism Microsomes, Liver/metabolism Nucleoside Diphosphate Sugars/metabolism Polyisoprenyl Phosphates/metabolism Rats Vitamin A/analogs & derivatives,metabolism

Chemicals

Diterpenes Membrane Lipids Membrane Proteins Nucleoside Diphosphate Sugars Polyisoprenyl Phosphates Vitamin A retinol phosphate

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Bergman A

Mankowski T

Chojnacki T

De Luca L M

Peterson E

Dallner G

References (13)

13 references, click to expand

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Chemical synthesis of all-trans-beta-retinoyl phosphat.
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Subcellular distribution of dolichol phosphate.
FEBS Lett. 1972 Aug 15;24(3):315-317 PMID: 11946696
Intracellular localization of liver sugar nucleotide glycoprotein glycosyltransferases in a Golgi-rich fraction.
J Biol Chem. 1970 Mar 10;245(5):1090-100 PMID: 4392041
Distribution of protein-bound sugar residues in microsomal subfractions and Golgi membranes.
Biochim Biophys Acta. 1976 May 21;433(3):496-508 PMID: 1276191
Isolation of rough and smooth microsomes--general.
Methods Enzymol. 1974;31:191-201 PMID: 4418022
C55-Dolichol: occurrence in pig liver and preparation by hydrogenation of plant undecaprenol.
Biochemistry. 1976 May 18;15(10):2125-30 PMID: 1276128
Golgi fractions prepared from rat liver homogenates. I. Isolation procedure and morphological characterization.
J Cell Biol. 1973 Oct;59(1):45-72 PMID: 4356571
The role of polyprenol-linked sugars in glycoprotein synthesis.
Annu Rev Biochem. 1976;45:95-112 PMID: 786163
Separation of mannosylretinylphosphate from dolichyl-mannosylphosphate by solvent extraction.
Anal Biochem. 1976 Oct;75(2):664-7 PMID: 984418
The transfer of mannose to dolichol diphosphate oligosaccharides in pig liver endoplasmic reticulum.
Biochem J. 1975 Nov;152(2):191-9 PMID: 1220679
The direct involvement of vitamin A in glycosyl transfer reactions of mammalian membranes.
Vitam Horm. 1977;35:1-57 PMID: 607563
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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1720123

Published

1978-04-15

Pages

123-7

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1185670

Subset

External Links

PubMed source DOI: 10.1042/bj1720123 PMC Full Text View journal details

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Glycosyl transfer from nucleotide sugars to C85- and C55-polyprenyl and retinyl phosphates by microsomal subfractions and Golgi membranes of rat liver.

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