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PMID: 6561521 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Lysine tRNAs from rat liver: lysine tRNA sequences are highly conserved.

Nucleic acids research ·Vol. 12 ·No. 5 ·1984-03-12 ·Pages 2535-41

Hedgcoth C, Hayenga K, Harrison M, Ortwerth BJ

Abstract

The two major lysine tRNAs from rat liver, tRNA2Lys and tRNA5Lys, were sequenced by rapid gel or chromatogram readout methods. The major tRNA2Lys differs from a minor form only by a base pair in positions 29 and 41; both tRNAs have an unidentified nucleotide, U**, in the third position of the anticodon. Although highly related, the major tRNA2Lys and tRNA5Lys differ in four base pairs and four unpaired nucleotides, including the first position of the anticodons, but have the same base pair in positions 29 and 41. The three tRNAs maintain a m2G-U pair in the acceptor stem. Detection of this m2G is in contrast to other reports of lysine tRNAs. Sequences of lysine tRNAs are strongly conserved in higher eukaryotes.

MeSH Terms
Animals Base Composition Base Sequence Chromatography, Gel Chromatography, Ion Exchange Liver/metabolism Nucleic Acid Conformation RNA, Transfer, Amino Acyl/genetics,isolation & purification Rats
Chemicals
RNA, Transfer, Amino Acyl
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hedgcoth C
Hayenga K
Harrison M
Ortwerth B J
References (26)
26 references, click to expand
  1. Isoaccepting transfer ribonucleic acids in specialized mammalian tissues.
    Biochemistry. 1971 Nov;10(23):4190-7 PMID: 5166641
  2. Minor components in transfer RNA: their characterization, location, and function.
    Prog Nucleic Acid Res Mol Biol. 1972;12:49-85 PMID: 4557059
  3. Correlation between a specific isoaccepting lysyl transfer ribonucleic acid and cell division in mammalian tissues.
    Biochemistry. 1973 Sep 25;12(20):3978-84 PMID: 4355545
  4. Purification of the four lysine-specific transfer ribonucleic acids from chick embryos.
    Biochim Biophys Acta. 1973 Dec 7;331(2):221-30 PMID: 4774407
  5. Amounts of isoaccepting lysine tRNAs change with the proliferative state of cells.
    Nature. 1975 Mar 27;254(5498):359-60 PMID: 1167943
  6. Relation of cell type and cell density to the degree of post-transcriptional modification of tRNALys and tRNAPhe.
    Biochim Biophys Acta. 1975 Nov 4;407(4):392-8 PMID: 170990
  7. Mapping adenines, guanines, and pyrimidines in RNA.
    Nucleic Acids Res. 1977 Aug;4(8):2527-38 PMID: 409999
  8. Sequence analysis of 5'[32P] labeled mRNA and tRNA using polyacrylamide gel electrophoresis.
    Nucleic Acids Res. 1978 Jan;5(1):37-56 PMID: 25417
  9. A different approach to RNA sequencing.
    Nature. 1978 Jul 6;274(5666):87-9 PMID: 662002
  10. The nucleotide sequence of lysine tRNA2 from Drosophila.
    Nucleic Acids Res. 1979 Feb;6(2):435-42 PMID: 106371
  11. Use of in vitro 32P labeling in the sequence analysis of nonradioactive tRNAs.
    Methods Enzymol. 1979;59:58-109 PMID: 220499
  12. Direct chemical method for sequencing RNA.
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):1760-4 PMID: 377283
  13. Nucleotide sequence of three isoaccepting lysine tRNAs from rabbit liver and SV40-transformed mouse fibroblasts.
    Eur J Biochem. 1979 Jun;97(1):305-18 PMID: 225173
  14. Rapid print-readout technique for sequencing of RNA's containing modified nucleotides.
    Nucleic Acids Res. 1979 Aug 10;6(11):3443-58 PMID: 386273
  15. The purification and properties of chicken liver RNase: An enzyme which is useful in distinguishing between cytidylic and uridylic acid residues.
    J Biol Chem. 1980 Mar 10;255(5):2153-9 PMID: 6243659
  16. Lys-tRNA levels and cell division in mouse 3T3 cells.
    Exp Cell Res. 1980 Jul;128(1):171-80 PMID: 6773786
  17. Chemical probes for higher-order structure in RNA.
    Proc Natl Acad Sci U S A. 1980 Aug;77(8):4679-82 PMID: 6159633
  18. Cell growth and tRNA-lys4 synthesis in mouse 3T3 cells.
    Exp Cell Res. 1982 Apr;138(2):241-50 PMID: 6281043
  19. Lysine tRNAs from Bacillus subtilis 168: structural analysis.
    Nucleic Acids Res. 1982 May 25;10(10):3125-32 PMID: 6808464
  20. The structure of tRNA 5 Lys from Drosophila melanogaster.
    Nucleic Acids Res. 1982 Oct 25;10(20):6393-9 PMID: 6817300
  21. A rat tRNA gene cluster containing the genes for tRNAPro and tRNALys. Analysis of nucleotide sequences of the genes and the surrounding regions.
    Nucleic Acids Res. 1982 Oct 25;10(20):6411-9 PMID: 6294603
  22. Nucleotide sequence of a segment of human DNA containing the three tRNA genes.
    Nucleic Acids Res. 1982 Nov 25;10(22):7313-22 PMID: 6296772
  23. Using iodinated single-stranded M13 probes to facilitate rapid DNA sequence analysis--nucleotide sequence of a mouse lysine tRNA gene.
    Nucleic Acids Res. 1983 Apr 11;11(7):2053-64 PMID: 6300790
  24. The effects of growth factors on tRNALys modification.
    Recent Results Cancer Res. 1983;84:160-70 PMID: 6342071
  25. Perturbation of the mitochondrial lysine tRNA population by virus-induced transformation or stress of mammalian cells: functional properties and nucleotide sequence of a mitochondrially associated lysine tRNA.
    Recent Results Cancer Res. 1983;84:171-83 PMID: 6342072
  26. Competence and progression growth factors stimulate different tRNAlys modification reactions in BALB/C 3T3 cells.
    Biochem Biophys Res Commun. 1983 Sep 15;115(2):598-605 PMID: 6354186
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.5.2535
Published
1984-03-12
Pages
2535-41
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318682
Subset
IM
Grants
NCI NIH HHS · CA12741 · United States
NCI NIH HHS · CA26423 · United States
Databases
GENBANK
X04545, X04546
External Links
PubMed source DOI: 10.1093/nar/12.5.2535 PMC Full Text View journal details
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