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PMID: 65719 Published · ppublish ger English Abstract Journal Article

[Staining the Nissl bodies in 4--10 microns thick Araldite sections with an area of about 2x2 cm (author's transl)].

Färbung der Nissl-Substanz in 4--10 micron dicken und 2x2 cm grossen Aralditschnitten

Microscopica acta ·Vol. 78 ·No. 4 ·1976-09-00 ·Pages 289-91

Braak E

Abstract

For light microscopy 4--10 microns thick sections with an area of 2x2 cm o Araldite-embedded tissue (human autopsy brain) are stained in 1% aqueous methylene blue at 65 degrees C for 4--12 hours and are differentiated in 98% ethanol for some seconds or very few minutes. The Nissl bodies, nuclei and nucleoli are stained dark blue, the neuropil is nearly colourless.

MeSH Terms
Cerebral Cortex/ultrastructure Fixatives Histological Techniques Humans Methylene Blue Nissl Bodies/ultrastructure Resins, Synthetic Staining and Labeling
Chemicals
Fixatives Resins, Synthetic Methylene Blue
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Braak E
Article Info
Journal
Microscopica acta
Abbr.
Microsc Acta
ISSN
0044-376X
Published
1976-09-00
Pages
289-91
Language
ger
Region
Germany
NLM ID
1306037
Subset
IM
External Links
PubMed source
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