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PMID: 6571989 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Metal and sulfur composition of iron-molybdenum cofactor of nitrogenase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 1 ·1983-01-00 ·Pages 147-50

Nelson MJ, Levy MA, Orme-Johnson WH

Abstract

The sulfur content of N-methylformamide solutions of cofactor from Clostridium pasteurianum nitrogenase has been determined to be 11.9 (+/- 0.9) mol per mol of molybdenum. This number was determined radiochemically, using iron-molybdenum cofactor isolated from molybdenum-iron protein from bacteria grown on 35SO4. A total of 3.2 (+/- 0.2) mol of sulfur per mol of molybdenum was found to be present in cysteine and methionine, probably arising from contaminating proteins not intrinsic to the cofactor. Combined with accumulated evidence that is discussed, these results lead to an updated stoichiometry of MoFe6S8 or 9, not MoFe6S4 as previously thought, for this cluster.

MeSH Terms
Bacterial Proteins/analysis Clostridium/analysis Iron/analysis Metalloproteins/analysis Molybdenum/analysis Nitrogenase/analysis Sulfur/analysis
Chemicals
Bacterial Proteins Metalloproteins Sulfur Molybdenum Iron Nitrogenase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Nelson M J
Levy M A
Orme-Johnson W H
References (15)
15 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.1.147
Published
1983-01-00
Pages
147-50
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC393327
Subset
IM
Grants
NIGMS NIH HHS · 1 F32 GM08710-01 · United States
NIGMS NIH HHS · 5 F32 GM07294 · United States
External Links
PubMed source DOI: 10.1073/pnas.80.1.147 PMC Full Text View journal details
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