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PMID: 6572921 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Human coagulation factor Va is a cofactor for the activation of protein C.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 6 ·1983-03-00 ·Pages 1584-8

Salem HH, Broze GJ, Miletich JP, Majerus PW

Abstract

During blood clotting in vitro, protein C is converted in part to protein Ca, Protein Ca, in turn, inactivates factor Va. This is evidenced by the rapid inactivation of factor Va coagulant activity after clot formation which is associated with the cleavage of the Mr 110,000 peptide of factor Va. When exogenous factor Va is added to serum, it is inactivated only after a lag of 10-20 min. Using purified coagulation factors in the presence of EDTA, we demonstrated that factor Va enhances the rate of protein C activation by thrombin by 50-fold. The Km for factor Va in the reaction is 14 nM, 100 times higher than its Km for accelerating platelet surface prothrombin activation by factor Xa. By this mechanism, factor Va can act as a procoagulant as well as limit dissemination of the coagulation process through the activation of protein C and the subsequent inactivation of both factor Va and factor VIIIa.

MeSH Terms
Blood Coagulation Blood Coagulation Factors/physiology Enzyme Activation Factor V/physiology Glycoproteins/physiology Humans In Vitro Techniques Peptide Fragments Protein C
Chemicals
Blood Coagulation Factors Glycoproteins Peptide Fragments Protein C Factor V
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Salem H H
Broze G J
Miletich J P
Majerus P W
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22 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.6.1584
Published
1983-03-00
Pages
1584-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC393646
Subset
IM
Grants
NHLBI NIH HHS · HL 16634 · United States
NHLBI NIH HHS · HLBI 14147 · United States
External Links
PubMed source DOI: 10.1073/pnas.80.6.1584 PMC Full Text View journal details
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