Separation of Fe+3 from transferrin in endocytosis. Role of the acidic endosome.

FEBS letters ·Vol. 160 ·No. 1-2 ·1983-08-22 ·Pages 213-6

Rao K, van Renswoude J, Kempf C, Klausner RD

Abstract

NH4Cl and monensin, two agents which neutralize intracellular acidic compartments, block the segregation of iron from transferrin after endocytosis, while neither of these reagents affects internalization of diferric transferrin into the cell. In conclusion the molecular separation of iron from transferrin inside the cell requires a non-lysosomal acidic compartment.

MeSH Terms

Ammonium Chloride/pharmacology Cell Line Endocytosis Humans Hydrogen-Ion Concentration Iron/metabolism Kinetics Leukemia, Myeloid, Acute Microbodies/drug effects,metabolism Monensin/pharmacology Organoids/metabolism Protein Binding Transferrin/metabolism

Chemicals

Transferrin Ammonium Chloride Monensin Iron

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Rao K

van Renswoude J

Kempf C

Klausner R D

Article Info

Journal

FEBS letters

Abbr.

FEBS Lett

ISSN

0014-5793

DOI

10.1016/0014-5793(83)80969-7

Published

1983-08-22

Pages

213-6

Language

English

Region

England

NLM ID

0155157

Subset

External Links

PubMed source DOI: 10.1016/0014-5793(83)80969-7 View journal details

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Separation of Fe+3 from transferrin in endocytosis. Role of the acidic endosome.

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