Home LiteratureArticle Details
PMID: 6579553 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Detection of hepatitis B virus DNA in hepatocytes, bile duct epithelium, and vascular elements by in situ hybridization.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 21 ·1983-11-00 ·Pages 6685-8

Blum HE, Stowring L, Figus A, Montgomery CK, Haase AT, Vyas GN

Abstract

A radiolabeled probe specific for hepatitis B virus (HBV) nucleotide sequences was hybridized in situ to liver tissue from three patients with chronic hepatitis B. The HBV genome was detected not only in infected hepatocytes but also in bile duct epithelial cells, endothelial cells, and smooth muscle cells. These findings extend the known host cell range for HBV, suggest new mechanisms of viral dissemination, and illustrate the usefulness of in situ hybridization in the study of pathogenesis of HBV infection.

MeSH Terms
Animals Bile Ducts/microbiology DNA, Viral/analysis Epithelium/microbiology Hepatitis B virus/analysis Humans Liver/blood supply,microbiology Mice Nucleic Acid Hybridization
Chemicals
DNA, Viral
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Blum H E
Stowring L
Figus A
Montgomery C K
Haase A T
Vyas G N
References (35)
35 references, click to expand
  1. Preparative and analytical purification of DNA from agarose.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):615-9 PMID: 284385
  2. Acute and chronic hepatitis revisited. Review by an international group.
    Lancet. 1977 Oct 29;2(8044):914-9 PMID: 72251
  3. Presence of integrated hepatitis B virus DNA sequences in cellular DNA of human hepatocellular carcinoma.
    Nature. 1980 Jul 31;286(5772):533-5 PMID: 6250074
  4. Integration of hepatitis B virus sequences and their expression in a human hepatoma cell.
    Nature. 1980 Jul 31;286(5772):535-8 PMID: 6250075
  5. State of hepatitis B viral DNA in a human hepatoma cell line.
    J Virol. 1980 Feb;33(2):795-806 PMID: 6251250
  6. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  7. Integration pattern of hepatitis B virus DNA sequences in human hepatoma cell lines.
    J Virol. 1981 Jan;37(1):239-43 PMID: 6260977
  8. Biology of hepatitis B virus.
    Science. 1981 Jul 24;213(4506):406-11 PMID: 6264599
  9. State of hepatitis B virus DNA in hepatocytes of patients with hepatitis B surface antigen-positive and -negative liver diseases.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3906-10 PMID: 6267609
  10. Integration of hepatitis B virus DNA into the genome of liver cells in chronic liver disease and hepatocellular carcinoma. Studies in percutaneous liver biopsies and post-mortem tissue specimens.
    N Engl J Med. 1981 Oct 29;305(18):1067-73 PMID: 6268980
  11. Light microscopic localization of hepatitis B virus antigens in the human pancreas. Possibility of multiplication of hepatitis B virus in the human pancreas.
    Gastroenterology. 1981 Dec;81(6):998-1005 PMID: 6169587
  12. Identification of integrated hepatitis B virus DNA sequences in human hepatocellular carcinomas.
    Hepatology. 1981 Jan-Feb;1(1):1-8 PMID: 6269974
  13. Quantitative analysis of steroid autoradiograms.
    J Histochem Cytochem. 1981 Jan;29(1A Suppl):207-11 PMID: 7288157
  14. Viral hepatitis: report on WHO informal consultation.
    J Med Virol. 1981;8(1):1-29 PMID: 6271920
  15. Detection of hepatitis B virus DNA sequences in infected hepatocytes by in situ cytohybridisation.
    J Med Virol. 1981;8(1):67-78 PMID: 7299375
  16. Detection of hepatitis B virus-specific DNA in the genomes of human hepatocellular carcinoma and liver cirrhosis tissues.
    J Gen Virol. 1981 Nov;57(Pt 1):95-102 PMID: 6275024
  17. Evidence that hepatitis B virus has a role in liver-cell carcinoma in alcoholic liver disease.
    N Engl J Med. 1982 Jun 10;306(23):1384-7 PMID: 6281640
  18. Visna DNA synthesis and the tempo of infection in vitro.
    Virology. 1982 Jun;119(2):399-410 PMID: 6281982
  19. Cytoplasmic dot hybridization. Simple analysis of relative mRNA levels in multiple small cell or tissue samples.
    J Biol Chem. 1982 Aug 10;257(15):8569-72 PMID: 7096324
  20. Evidence for non-chromosomal hepatitis B virus surface (HBsAg)- and core antigen (HBcAg)-specific DNA sequences in a hepatoma cell line.
    J Gen Virol. 1982 Jul;61 (Pt l):105-9 PMID: 6288845
  21. Characterization of integrated hepatitis B viral DNA cloned from a human hepatoma and the hepatoma-derived cell line PLC/PRF/5.
    Proc Natl Acad Sci U S A. 1983 May;80(9):2505-9 PMID: 6302689
  22. Integrated hepatitis B virus DNA sequences specifying the major viral core polypeptide are methylated in PLC/PRF/5 cells.
    Proc Natl Acad Sci U S A. 1983 May;80(9):2534-8 PMID: 6302693
  23. Viral nucleic acid synthesis and antigen accumulation in pancreas and kidney of Pekin ducks infected with duck hepatitis B virus.
    Proc Natl Acad Sci U S A. 1983 Aug;80(15):4865-9 PMID: 6576362
  24. Hepatitis-associated antigen in human bile.
    Lancet. 1971 Dec 4;2(7736):1266 PMID: 4143627
  25. Tissue localization of Australia antigen immune complexes in acute and chronic hepatitis and liver cirrhosis.
    Am J Pathol. 1972 Jul;68(1):31-56 PMID: 4628111
  26. The role of circulating hepatitis B antigen/antibody immune complexes in the pathogenesis of vascular and hepatic manifestations in polyarteritis nodosa.
    J Clin Pathol. 1974 Nov;27(11):863-8 PMID: 4155412
  27. Presence of HBsAg in the bile of subjects with acute HBsAg viral hepatitis.
    Pathol Microbiol (Basel). 1975;43(4):307-16 PMID: 1228594
  28. Hepatitis B surface antigen produced by a human hepatoma cell line.
    Br J Cancer. 1976 Nov;34(5):509-15 PMID: 187208
  29. Ecdysone-stimulated RNA synthesis in imaginal discs of Drosophila melanogaster. Assay by in situ hybridization.
    Chromosoma. 1976 Oct 12;58(1):87-99 PMID: 826375
  30. Slow persistent infection caused by visna virus: role of host restriction.
    Science. 1977 Jan 14;195(4274):175-7 PMID: 188133
  31. Establishment of a continuously growing cell line from primary carcinoma of the liver.
    S Afr Med J. 1976 Dec 18;50(54):2124-8 PMID: 63998
  32. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  33. The genome of hepatitis B virus.
    Annu Rev Microbiol. 1977;31:357-77 PMID: 334041
  34. Detection of viral sequences of low reiteration frequency by in situ hybridization.
    Proc Natl Acad Sci U S A. 1978 Dec;75(12):6125-9 PMID: 216014
  35. Identification of integrated hepatitis B virus DNA and expression of viral RNA in an HBsAg-producing human hepatocellular carcinoma cell line.
    Nature. 1980 Jul 31;286(5772):531-3 PMID: 6250073
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.21.6685
Published
1983-11-00
Pages
6685-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391235
Subset
IM
Grants
NIADDK NIH HHS · AM-26743 · United States
External Links
PubMed source DOI: 10.1073/pnas.80.21.6685 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2