Home LiteratureArticle Details
PMID: 6582473 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Perpendicular orientation and directional migration of amphibian neural crest cells in dc electrical fields.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 1 ·1984-01-00 ·Pages 160-4

Cooper MS, Keller RE

Abstract

The behavior of cultured neural crest cells of Ambystoma mexicanum and Xenopus laevis in dc electrical fields was studied. In fields of 1-5 V/cm, isolated or confluent cells retract both their anode- and cathode-facing margins. Subsequently, the cells elongate, with protrusive activity confined to their narrow ends. In larger fields (greater than or equal to 5 V/cm), protrusions form on the cathode-facing sides of the perpendicularly oriented cells. The cells then begin migrating laterally, perpendicular to their long axes, towards the cathode. We suggest that the perpendicular alignment and cathode-directed migrations result from cytoskeletal changes mediated by modified ion fluxes through the anode-facing (hyperpolarized) and cathode-facing (depolarized) cell membranes. The breaking of cellular confluence in response to dc electric fields is also discussed.

MeSH Terms
Ambystoma Animals Cell Movement Cells, Cultured Chemotaxis Electrophysiology Embryo, Nonmammalian Membrane Potentials Neural Crest/physiology
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cooper M S
Keller R E
References (22)
22 references, click to expand
  1. Effects of electrode placement on stimulation of adult frog limb regeneration.
    Ann N Y Acad Sci. 1974;238:500-7 PMID: 4548335
  2. Embryonic cell motility can be guided by physiological electric fields.
    Exp Cell Res. 1983 Aug;147(1):195-201 PMID: 6617761
  3. Electrical controls of development.
    Annu Rev Biophys Bioeng. 1977;6:445-76 PMID: 326151
  4. Bioelectricity and regeneration. I. Initiation of frog limb regeneration by minute currents.
    J Exp Zool. 1977 Jun;200(3):403-16 PMID: 301554
  5. Relations between ameboid movement and membrane-controlled electrical currents.
    J Gen Physiol. 1977 Jun;69(6):743-63 PMID: 19555
  6. Electrophoretic movement and localisation of acetylcholine receptors in the embryonic muscle cell membrane.
    Nature. 1978 Sep 7;275(5675):31-5 PMID: 683340
  7. Cytoplasmic structure and contractility in amoeboid cells.
    Int Rev Cytol. 1979;56:57-144 PMID: 37189
  8. Neurites grow faster towards the cathode than the anode in a steady field.
    J Exp Zool. 1979 Jul;209(1):115-28 PMID: 490126
  9. Ion channels in development.
    Annu Rev Neurosci. 1979;2:363-97 PMID: 395884
  10. Analysis of the neural crest ventral pathway using injected tracer cells.
    Dev Biol. 1980 Jun 1;77(1):130-41 PMID: 7399115
  11. Analysis of migratory behavior of neural crest and fibroblastic cells in embryonic tissues.
    Dev Biol. 1980 Jun 1;77(1):142-56 PMID: 7399116
  12. Fibroblast traction as a mechanism for collagen morphogenesis.
    Nature. 1981 Mar 19;290(5803):249-51 PMID: 7207616
  13. Fibronectin is not present in the focal adhesions formed between normal cultured fibroblasts and their substrata.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7318-22 PMID: 6938979
  14. In situ electrophoresis of membrane components.
    Annu Rev Biophys Bioeng. 1981;10:245-76 PMID: 7020576
  15. The direction of growth of differentiating neurones and myoblasts from frog embryos in an applied electric field.
    J Physiol. 1981 May;314:121-35 PMID: 7310685
  16. The glabrous epidermis of cavies contains a powerful battery.
    Am J Physiol. 1982 Mar;242(3):R358-66 PMID: 7065232
  17. Orientation of neurite growth by extracellular electric fields.
    J Neurosci. 1982 Apr;2(4):483-96 PMID: 6279799
  18. Measurement of intracellular free calcium in monkey kidney cells with aequorin.
    Science. 1982 Jul 16;217(4556):252-4 PMID: 6806904
  19. What is the role of naturally produced electric current in vertebrate regeneration and healing.
    Int Rev Cytol. 1982;76:245-98 PMID: 6749746
  20. Directional protrusive pseudopodial activity and motility in macrophages induced by extracellular electric fields.
    Cell Motil. 1982;2(3):243-55 PMID: 6816471
  21. Changes in cell shape and actin distribution induced by constant electric fields.
    Nature. 1983 May 5-11;303(5912):61-4 PMID: 6682488
  22. Electrophoresis along cell membranes.
    Nature. 1977 Feb 17;265(5595):600-2 PMID: 859558
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.1.160
Published
1984-01-00
Pages
160-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC344630
Subset
IM
Grants
NIGMS NIH HHS · 1-T32-GM07379 · United States
External Links
PubMed source DOI: 10.1073/pnas.81.1.160 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2