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PMID: 6582476 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A molecular approach to leukemogenesis: mouse lymphomas contain an activated c-ras oncogene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 1 ·1984-01-00 ·Pages 202-5

Guerrero I, Calzada P, Mayer A, Pellicer A

Abstract

By inducing mouse thymomas with carcinogens and gamma-radiation, we have studied the potential of tumor DNA to induce foci in rodent fibroblasts. A high percentage of the tumors used transformed the cultured cells, and the oncogenic phenotype segregated with extra copies of the c-ras gene family. There appears to be selectivity in the activated gene because so far all analyzed tumors induced by carcinogen have activated the N-ras gene, and those induced by radiation have activated the K-ras gene. The K-ras gene is the cellular counterpart of the viral ras oncogene in Kirsten murine sarcoma virus, but the N-ras has not yet been found in a retrovirus. The transformed cells have a marked increase in expression of the oncogene at the RNA and protein level. This model system might be a powerful tool in the study of leukemogenesis.

MeSH Terms
Animals Carcinogens Cell Transformation, Neoplastic Cells, Cultured DNA, Neoplasm/genetics,isolation & purification Leukemia, Experimental/genetics Leukemia, Radiation-Induced/genetics Mice Oncogenes/radiation effects RNA, Neoplasm/isolation & purification Thymoma/genetics Thymus Neoplasms/genetics Transcription, Genetic
Chemicals
Carcinogens DNA, Neoplasm RNA, Neoplasm
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Guerrero I
Calzada P
Mayer A
Pellicer A
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35 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.1.202
Published
1984-01-00
Pages
202-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC344639
Subset
IM
Grants
NCI NIH HHS · CA-16239 · United States
External Links
PubMed source DOI: 10.1073/pnas.81.1.202 PMC Full Text View journal details
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