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PMID: 6583695 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

3' editing of mRNAs: sequence requirements and involvement of a 60-nucleotide RNA in maturation of histone mRNA precursors.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 4 ·1984-02-00 ·Pages 1057-61

Birchmeier C, Schümperli D, Sconzo G, Birnstiel ML

Abstract

In vitro-synthesized transcripts of the sea urchin histone H2A gene with 3' extensions are efficiently and rapidly processed to H2A mRNA with faithful 3' ends in Xenopus laevis oocyte nuclei. Processing requires the presence of a histone-specific dyad symmetry element and of H2A-proximal spacer sequences in the precursor RNA. In DNA injection experiments with a processing-deficient H2A mutant, the transcription products appear to terminate heterogeneously in the first 100-200 base pairs of the post-H2A spacer. Processing of synthetic H3 RNA precursors requires the prior injection of a 60-nucleotide RNA from sea urchin embryos that seems to be a component of a small nuclear ribonucleoprotein.

MeSH Terms
Animals Base Composition Base Sequence DNA, Recombinant/metabolism Female Histones/genetics Mutation Oocytes/metabolism Protein Biosynthesis RNA Processing, Post-Transcriptional RNA, Messenger/genetics Transcription, Genetic Xenopus
Chemicals
DNA, Recombinant Histones RNA, Messenger
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Birchmeier C
Schümperli D
Sconzo G
Birnstiel M L
References (21)
21 references, click to expand
  1. Mapping of RNA by a modification of the Berk-Sharp procedure: the 5' termini of 15 S beta-globin mRNA precursor and mature 10 s beta-globin mRNA have identical map coordinates.
    Nucleic Acids Res. 1979 Nov 10;7(5):1175-93 PMID: 390497
  2. Are snRNPs involved in splicing?
    Nature. 1980 Jan 10;283(5743):220-4 PMID: 7350545
  3. Transcriptional fidelity of histone genes injected into Xenopus oocyte nuclei.
    Nature. 1980 Nov 6;288(5786):100-2 PMID: 7432506
  4. The sequence 5'-AAUAAA-3'forms parts of the recognition site for polyadenylation of late SV40 mRNAs.
    Cell. 1981 Apr;24(1):251-60 PMID: 6113054
  5. Differential translation efficiency explains discoordinate expression of the galactose operon.
    Cell. 1981 Jul;25(1):241-9 PMID: 7023696
  6. The organization and expression of histone gene families.
    Cell. 1981 Aug;25(2):301-13 PMID: 6793234
  7. Plasmid vectors for high-efficiency expression controlled by the PL promoter of coliphage lambda.
    Gene. 1981 Oct;15(1):81-93 PMID: 6271633
  8. Variety in the level of gene control in eukaryotic cells.
    Nature. 1982 Jun 3;297(5865):365-71 PMID: 6176879
  9. Generation of authentic 3' termini of an H2A mRNA in vivo is dependent on a short inverted DNA repeat and on spacer sequences.
    Cell. 1982 Apr;28(4):739-45 PMID: 6284372
  10. Bioassay for components regulating eukaryotic gene expression: a chromosomal factor involved in the generation of histone mRNA 3' termini.
    Proc Natl Acad Sci U S A. 1982 Oct;79(20):6201-4 PMID: 6959109
  11. Simple, efficient in vitro synthesis of capped RNA useful for direct expression of cloned eukaryotic genes.
    Nucleic Acids Res. 1982 Oct 25;10(20):6353-62 PMID: 6294601
  12. Transcription of sea-urchin mesenchyme blastula histone genes after heat shock.
    Eur J Biochem. 1982 Nov 15;128(2-3):509-13 PMID: 7151793
  13. Human beta-globin pre-mRNA synthesized in vitro is accurately spliced in Xenopus oocyte nuclei.
    Cell. 1983 Mar;32(3):681-94 PMID: 6550524
  14. The U1 small nuclear RNA-protein complex selectively binds a 5' splice site in vitro.
    Cell. 1983 Jun;33(2):509-18 PMID: 6190573
  15. Inhibition of RNA cleavage but not polyadenylation by a point mutation in mRNA 3' consensus sequence AAUAAA.
    Nature. 1983 Oct 13-19;305(5935):600-5 PMID: 6194440
  16. Biochemical complementation with RNA in the Xenopus oocyte: a small RNA is required for the generation of 3' histone mRNA termini.
    Cell. 1983 Oct;34(3):823-8 PMID: 6194891
  17. The terminal RNA stem-loop structure and 80 bp of spacer DNA are required for the formation of 3' termini of sea urchin H2A mRNA.
    Cell. 1983 Dec;35(2 Pt 1):433-40 PMID: 6317188
  18. Monocistronic transcription is the physiological mechanism of sea urchin embryonic histone gene expression.
    Mol Cell Biol. 1981 Jul;1(7):661-71 PMID: 9279379
  19. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
    Cell. 1977 Nov;12(3):721-32 PMID: 922889
  20. Histone genes and histone messengers.
    Annu Rev Biochem. 1979;48:837-70 PMID: 112914
  21. Expression of sea urchin histone genes in the oocyte of Xenopus laevis.
    J Mol Biol. 1979 Dec 15;135(3):709-32 PMID: 537089
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.4.1057
Published
1984-02-00
Pages
1057-61
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC344763
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.81.4.1057 PMC Full Text View journal details
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