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PMID: 6583696 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Adaptive response in mammalian cells: crossreactivity of different pretreatments on cytotoxicity as contrasted to mutagenicity.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 4 ·1984-02-00 ·Pages 1062-6

Laval F, Laval J

Abstract

Pretreatment of H4 (rat hepatoma) cells for 48 hr with low nontoxic doses of alkylating agents [methyl methanesulfonate (MMS), N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), and N-methyl-N-nitrosourea] renders the cells more resistant to the toxic effect of these compounds. Crossreactivity for survival is also observed with the different alkylating agents tested. Pretreatment with MNNG enables the cells to be less mutated than control cultures during a subsequent challenge with high doses of this compound. However, pretreatment with MMS does not modify the mutation frequency of cells challenged with either MMS or MNNG. The adaptive response to mutagenesis is correlated with a faster and more efficient removal of O6-methylguanine in MNNG-pretreated cells as compared to control cultures, whereas the disappearance of this lesion is not modified in MMS-pretreated cells. As MMS produces less methylation at the O6 position of guanine and more methylation at the N7 position in comparison to MNNG, the results suggest that: (i) N7-methylguanine is not implicated in the adaptive response and (ii) adaptation to mutagenesis can be correlated with the amount of O6-methylguanine induced during the pretreatment. The effect of pretreatment on other O-alkylated derivatives is not known.

MeSH Terms
Acclimatization Alkylating Agents/toxicity Animals Cell Line Cell Survival/drug effects Drug Resistance Kinetics Liver Neoplasms, Experimental/physiopathology Methyl Methanesulfonate/toxicity Methylnitronitrosoguanidine/toxicity Methylnitrosourea/toxicity Mutagens Mutation Rats
Chemicals
Alkylating Agents Mutagens Methylnitronitrosoguanidine Methylnitrosourea Methyl Methanesulfonate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Laval F
Laval J
References (36)
36 references, click to expand
  1. Repair processes and the response of dividing and non-dividing cells to methyl methanesulphonate and dimethyl sulphate.
    Chem Biol Interact. 1976 Oct 2;15(2):117-30 PMID: 184969
  2. The chemical effects of nucleic acid alkylation and their relation to mutagenesis and carcinogenesis.
    Prog Nucleic Acid Res Mol Biol. 1975;15(0):219-84 PMID: 237307
  3. Enhancement of survival of X-irradiated mammalian cells by the uncoupler of oxidative phosphorylation, m-chloro carbonyl cyanide phenylhydrazone.
    Radiat Res. 1977 Sep;71(3):571-8 PMID: 897084
  4. An adaptive response of E. coli to low levels of alkylating agent: comparison with previously characterised DNA repair pathways.
    Mol Gen Genet. 1977 Nov 29;157(1):1-9 PMID: 414071
  5. Alkylation of deoxyribonucleic acid in vivo in various organs of C57BL mice by the carcinogens N-methyl-N-nitrosourea, N-ethyl-N-nitrosourea and ethyl methanesulphonate in relation to induction of thymic lymphoma. Some applications of high-pressure liquid chromatography.
    Biochem J. 1978 Sep 15;174(3):1031-44 PMID: 728073
  6. Increased excision of O6-methylguanine from rat liver DNA after chronic administration of dimethylnitrosamine.
    Cancer Res. 1979 May;39(5):1798-802 PMID: 427812
  7. Effect of uncouplers on radiosensitivity and mutagenicity in x-irradiated mammalian cells.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2702-5 PMID: 6930660
  8. Repair of alkylated DNA in Escherichia coli. Methyl group transfer from O6-methylguanine to a protein cysteine residue.
    J Biol Chem. 1980 Nov 25;255(22):10569-71 PMID: 7000780
  9. Evidence for an adaptive DNA repair pathway in CHO and human skin fibroblast cell lines.
    Nature. 1980 Oct 30;287(5785):861-3 PMID: 7432501
  10. Differential repair of O(6)-methylguanine in DNA of rat hepatocytes and nonparenchymal cells.
    Nature. 1980 Nov 13;288(5787):185-41 PMID: 7432520
  11. Cycle-related toxicity and transformation in 10T1/2 cells treated with N-methyl-N'-nitro-N-nitrosoguanidine.
    Proc Natl Acad Sci U S A. 1980 Aug;77(8):4813-7 PMID: 6933531
  12. Isolation of mutants of Escherichia coli with increased resistance to alkylating agents: mutants deficient in thiols and mutants constitutive for the adaptive response.
    Mol Gen Genet. 1980;180(1):85-90 PMID: 7003313
  13. Time course of O6-methylguanine removal from DNA of N-methyl-N-nitrosourea-treated human fibroblasts.
    Nature. 1981 Feb 26;289(5800):796-8 PMID: 7464943
  14. Demethylation of O6-methylguanine in a synthetic DNA polymer by an inducible activity in Escherichia coli.
    Biochem Biophys Res Commun. 1980 Nov 28;97(2):654-9 PMID: 7008792
  15. Kinetic analysis of O6-ethylguanine disappearance from DNA catalyzed by the chromatin factor of rat liver.
    FEBS Lett. 1980 Dec 29;122(2):271-4 PMID: 7202718
  16. Release of 7-methylguanine residues from alkylated DNA by extracts of Micrococcus luteus and Escherichia coli.
    Proc Natl Acad Sci U S A. 1981 Feb;78(2):852-5 PMID: 7015333
  17. Human lymphoblasts contain DNA glycosylase activity excising N-3 and N-7 methyl and ethyl purines but not O6-alkylguanines or 1-alkyladenines.
    Proc Natl Acad Sci U S A. 1981 Feb;78(2):856-60 PMID: 6940152
  18. Enzymatic release of 7-methylguanine from methylated DNA by rodent liver extracts.
    Proc Natl Acad Sci U S A. 1981 Feb;78(2):861-5 PMID: 7015334
  19. Pretreatment of Chinese hamster v79 cells with MNU increases survival without affecting DNA repair or mutagenicity.
    Carcinogenesis. 1981;2(1):55-60 PMID: 7273289
  20. Mode of action of methylating carcinogens: comparative studies of murine and human cells.
    Carcinogenesis. 1981;2(5):403-11 PMID: 7273321
  21. The inducible repair of alkylated DNA.
    Prog Nucleic Acid Res Mol Biol. 1981;26:237-44 PMID: 7025094
  22. Limited capacity for the removal of O6-methylguanine and its regeneration in a human lymphoma line.
    Carcinogenesis. 1981;2(12):1293-8 PMID: 7326830
  23. Alkylation of DNA and tissue specificity in nitrosamine carcinogenesis.
    J Supramol Struct Cell Biochem. 1981;17(3):259-73 PMID: 7328674
  24. Uracil-DNA glycosylase. Purification and properties of uracil-DNA glycosylase from Micrococcus luteus.
    J Biol Chem. 1982 Apr 10;257(7):3477-83 PMID: 7061492
  25. Enhanced survival and reduced mutation and aberration frequencies induced in V79 chinese hamster cells pre-exposed to low levels of methylating agents.
    Mutat Res. 1982 Mar;93(1):195-211 PMID: 7062931
  26. Induction of a DNA glycosylase for N-methylated purines is part of the adaptive response to alkylating agents.
    Nature. 1982 Apr 22;296(5859):770-3 PMID: 7040983
  27. Adaptation to alkylation resistance involves the induction of a DNA glycosylase.
    Nature. 1982 Apr 22;296(5859):773-5 PMID: 7040984
  28. Two DNA glycosylases in Escherichia coli which release primarily 3-methyladenine.
    Biochemistry. 1982 Mar 16;21(6):1162-9 PMID: 7041972
  29. Chemical mutagenesis.
    Annu Rev Biochem. 1982;51:655-93 PMID: 7051963
  30. Adaptive increase of O6-methylguanine-acceptor protein in HeLa cells following N-methyl-N'-nitro-N-nitrosoguanidine treatment.
    Nucleic Acids Res. 1982 Aug 11;10(15):4595-604 PMID: 7133992
  31. Repair of alkylated DNA in Escherichia coli. Physical properties of O6-methylguanine-DNA methyltransferase.
    J Biol Chem. 1982 Nov 25;257(22):13776-80 PMID: 6754717
  32. Purification and properties of O6-methylguanine-DNA transmethylase from rat liver.
    J Biol Chem. 1983 Feb 25;258(4):2327-33 PMID: 6822564
  33. Mutation induction in Chinese hamster ovary cells after chronic pretreatment with MNNG.
    Mutat Res. 1983 Mar;119(3):393-7 PMID: 6681863
  34. Tissue sulfhydryl groups.
    Arch Biochem Biophys. 1959 May;82(1):70-7 PMID: 13650640
  35. Methylation of deoxyribonucleic acid in cultured mammalian cells by N-methyl-N'-nitro-N-nitrosoguanidine. The influence of cellular thiol concentrations on the extent of methylation and the 6-oxygen atom of guanine as a site of methylation.
    Biochem J. 1970 Feb;116(4):693-707 PMID: 5435496
  36. A new pathway for DNA repair in Escherichia coli.
    Nature. 1977 May 19;267(5608):281-3 PMID: 325420
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.4.1062
Published
1984-02-00
Pages
1062-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC344764
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.81.4.1062 PMC Full Text View journal details
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