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PMID: 6583707 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Induction of two genes by glucose starvation in hamster fibroblasts.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 4 ·1984-02-00 ·Pages 988-92

Lin AY, Lee AS

Abstract

The coordinated expression of two genes specifically induced by glucose starvation is demonstrated in a hamster fibroblast cell line, K12. Using two cDNA plasmids, p4A3 and p3C5, as hybridization probes, we examine the kinetics of induction of these genes when the cells are grown in medium deprived of glucose. The results show that (i) after a lag period of about 8 hr, there is a rapid and simultaneous increase of the p4A3 and p3C5 mRNA levels and (ii) the elevation of the mRNA levels for p4A3 and p3C5 is largely due to new transcription. In addition, we compare the mRNA transcripts encoded by these glucose-regulated genes in culture cells and phosphoenolpyruvate carboxykinase, the enzyme that catalyzes the rate-limiting step in gluconeogenesis in fasted rats. Our results indicate that the expression of phosphoenolpyruvate carboxykinase is not inducible by glucose starvation in our culture cells.

MeSH Terms
Animals Cell Line Cloning, Molecular Cricetinae Cricetulus DNA/analysis Enzyme Induction Fibroblasts/metabolism Gene Amplification Genes Gluconeogenesis Glucose/metabolism Kinetics Lung Phosphoenolpyruvate Carboxykinase (GTP)/genetics RNA, Messenger/genetics Transcription, Genetic
Chemicals
RNA, Messenger DNA Phosphoenolpyruvate Carboxykinase (GTP) Glucose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lin A Y
Lee A S
References (19)
19 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.4.988
Published
1984-02-00
Pages
988-92
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC344748
Subset
IM
Grants
NCI NIH HHS · CA-27607 · United States
External Links
PubMed source DOI: 10.1073/pnas.81.4.988 PMC Full Text View journal details
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