Use of Monte Carlo calculations in the study of microtubule subunit kinetics.

GTP-tubulin forms a cap on microtubule ends during aggregation. The bulk of the microtubule is GDP-tubulin. This complicates the usual simple kinetic theory of subunit exchange at microtubule ends to such an extent that Monte Carlo calculations are needed to handle the complications, except in special cases. The Monte Carlo method is introduced here, for this problem, and illustrated with steady-state and transient examples. Monte Carlo transients are needed to simulate dilution experiments. Preliminary results (with M. F. Carlier) have been obtained applying these theoretical procedures to experimental data.