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PMID: 6584906 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Nucleotide sequence of the tms genes of the pTiA6NC octopine Ti plasmid: two gene products involved in plant tumorigenesis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 6 ·1984-03-00 ·Pages 1728-32

Klee H, Montoya A, Horodyski F, Lichtenstein C, Garfinkel D, Fuller S, Flores C, Peschon J, Nester E, Gordon M

Abstract

The nucleotide sequence of the tumor morphology locus, tms, from pTiA6NC has been determined. The sequence analysis indicates that each of two polyadenylylated transcripts encoded by this locus contains an open reading frame; the predicted transcript 1 gene product has a molecular size of 83,769 daltons, and the predicted transcript 2 gene product, of 49,588 daltons. The precise start and stop positions of the transcript 2 RNA have been mapped with S1 nuclease. Several insertion mutations have been constructed. One of these localizes the transcript 2 promoter within the 72 base pairs 5' to transcription initiation. Significant homology was observed between the protein encoded by transcript 1 and the adenine binding region of p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens, suggesting that the transcript 1 protein binds adenine either as substrate or cofactor.

MeSH Terms
Amino Acid Sequence Base Sequence DNA, Bacterial/genetics Genes Mutation Plant Tumors/genetics Plasmids RNA, Messenger/genetics Rhizobium/genetics
Chemicals
DNA, Bacterial RNA, Messenger
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Klee H
Montoya A
Horodyski F
Lichtenstein C
Garfinkel D
Fuller S
Flores C
Peschon J
Nester E
Gordon M
References (30)
30 references, click to expand
  1. Regulatory sequences involved in the promotion and termination of RNA transcription.
    Annu Rev Genet. 1979;13:319-53 PMID: 94251
  2. Nucleotide sequence and transcript map of the Agrobacterium tumefaciens Ti plasmid-encoded octopine synthase gene.
    J Mol Appl Genet. 1982;1(6):499-511 PMID: 7153687
  3. Cytokinin/auxin balance in crown gall tumors is regulated by specific loci in the T-DNA.
    Proc Natl Acad Sci U S A. 1983 Jan;80(2):407-11 PMID: 16593270
  4. The conserved part of the T-region in Ti-plasmids expresses four proteins in bacteria.
    EMBO J. 1983;2(3):403-9 PMID: 11894956
  5. T-DNA of the Agrobacterium Ti and Ri plasmids.
    Annu Rev Genet. 1982;16:357-84 PMID: 6297376
  6. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 PMID: 4504350
  7. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.
    J Bacteriol. 1972 May;110(2):667-76 PMID: 4336693
  8. Transcriptional control regions: nucleotide sequence requirements for initiation by RNA polymerase II and III.
    Curr Top Microbiol Immunol. 1981;93:25-46 PMID: 7026181
  9. The functional organization of the octopine Agrobacterium tumefaciens plasmid pTiB6s3.
    Plasmid. 1981 Sep;6(2):235-48 PMID: 6272338
  10. Grown gall plant tumors of abnormal morphology, induced by Agrobacterium tumefaciens carrying mutated octopine Ti plasmids; analysis of T-DNA functions.
    Gene. 1981 Jun-Jul;14(1-2):33-50 PMID: 6266929
  11. Integration and organization of Ti plasmid sequences in crown gall tumors.
    Cell. 1980 Mar;19(3):729-39 PMID: 7363328
  12. Plasmids specifying plant hyperplasias.
    Annu Rev Microbiol. 1981;35:531-65 PMID: 7027906
  13. Crystal structure of p-hydroxybenzoate hydroxylase.
    J Mol Biol. 1979 Jun 15;131(1):55-73 PMID: 40036
  14. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
    Gene. 1982 Oct;19(3):269-76 PMID: 6295880
  15. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
  16. Agrobacterium tumefaciens mutants affected in crown gall tumorigenesis and octopine catabolism.
    J Bacteriol. 1980 Nov;144(2):732-43 PMID: 6253441
  17. The TL-DNA in octopine crown-gall tumours codes for seven well-defined polyadenylated transcripts.
    EMBO J. 1982;1(1):139-46 PMID: 16453403
  18. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  19. Sizes and map positions of several plasmid-DNA-encoded transcripts in octopine-type crown gall tumors.
    Proc Natl Acad Sci U S A. 1982 Jan;79(1):76-80 PMID: 6275392
  20. Genetic Identification of functions of TL-DNA transcripts in octopine crown galls.
    EMBO J. 1982;1(1):147-52 PMID: 16453404
  21. Structural analysis of Tn5.
    Cold Spring Harb Symp Quant Biol. 1981;45 Pt 1:107-13 PMID: 6271452
  22. Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles.
    Microbiol Rev. 1983 Mar;47(1):1-45 PMID: 6343825
  23. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 PMID: 1105573
  24. Agrobacterium tumefaciens DNA and PS8 bacteriophage DNA not detected in crown gall tumors.
    Proc Natl Acad Sci U S A. 1974 Sep;71(9):3672-6 PMID: 4530328
  25. Characterization of Tn904 insertions in octopine Ti plasmid mutants of Agrobacterium tumefaciens.
    J Bacteriol. 1980 Oct;144(1):82-91 PMID: 6252198
  26. Identification of sequences involved in the polyadenylation of higher plant nuclear transcripts using Agrobacterium T-DNA genes as models.
    EMBO J. 1983;2(3):419-26 PMID: 11894958
  27. Interactions of phenolic acids, metallic ions and chelating agents on auxin-induced growth.
    Plant Physiol. 1966 Nov;41(9):1443-54 PMID: 16656422
  28. Genetic analysis of crown gall: fine structure map of the T-DNA by site-directed mutagenesis.
    Cell. 1981 Nov;27(1 Pt 2):143-53 PMID: 6276020
  29. Organization and expression of eucaryotic split genes coding for proteins.
    Annu Rev Biochem. 1981;50:349-83 PMID: 6791577
  30. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
    J Mol Biol. 1980 Oct 25;143(2):161-78 PMID: 6260957
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.6.1728
Published
1984-03-00
Pages
1728-32
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC344992
Subset
IM
Grants
NCI NIH HHS · CA 07249-01 · United States
Databases
GENBANK
K02553, K02554
External Links
PubMed source DOI: 10.1073/pnas.81.6.1728 PMC Full Text View journal details
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