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PMID: 6585825 Published · ppublish English Journal Article

Sequence of a cDNA clone encoding mouse glial fibrillary acidic protein: structural conservation of intermediate filaments.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 9 ·1984-05-00 ·Pages 2743-6

Lewis SA, Balcarek JM, Krek V, Shelanski M, Cowan NJ

Abstract

A clone encoding mouse glial fibrillary acidic protein (GFAP) was isolated from a cDNA library constructed so as to express the cloned sequences. The library was screened using a GFAP-specific polyclonal antiserum; a single bacterial colony expressing GFAP was identified. The complete sequence of the cDNA insert in this clone is presented, encompassing 2.5 kilobases and specifying greater than 97% of the GFAP amino acid sequence. The clone includes a long (1.4-kilobase) 3' untranslated region. Within the coding region, the data show extensive homology with other intermediate filament proteins, particularly in those regions predicted to be alpha-helical. RNA blot transfer experiments using the cloned GFAP cDNA probe revealed a single GFAP mRNA species of 2.7 kilobases in mouse brain. Southern blot analysis indicates the existence of at most two genes encoding GFAP in the mouse genome. The mouse GFAP probe cross-hybridizes weakly at high stringency with genomic DNA from diverse eukaryotic species.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Biological Evolution DNA/genetics Intermediate Filament Proteins/genetics Mice Neuroglia/physiology RNA, Messenger/genetics
Chemicals
Intermediate Filament Proteins RNA, Messenger DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lewis S A
Balcarek J M
Krek V
Shelanski M
Cowan N J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.9.2743
Published
1984-05-00
Pages
2743-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC345146
Subset
IM
Databases
GENBANK
K01347
External Links
PubMed source DOI: 10.1073/pnas.81.9.2743 PMC Full Text View journal details
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