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PMID: 6587321 Published · ppublish English Journal Article

CG dinucleotide clusters in MHC genes and in 5' demethylated genes.

Nucleic acids research ·Vol. 12 ·No. 10 ·1984-05-25 ·Pages 4385-96

Tykocinski ML, Max EE

Abstract

In the DNA of higher vertebrates the dinucleotide CG is unique in two respects: it occurs far less frequently than would be expected on the basis of the content of cytosine and guanine in a given DNA segment ("CG suppression") and it contains predominantly 5-methyl-cytosine, the only modified nucleotide common in vertebrate DNA. Here we point out the existence of CG clusters, i.e. localized lapses in the usual CG suppression, in two categories of DNA segments from vertebrates: around the polymorphic exons of major histocompatibility complex (MHC) genes and in the 5' regions of certain other genes. These observations contradict the recent suggestion that CG frequency is uniform over long contiguous segments of DNA containing several genes. A model for the origin of these CG clusters as a consequence of regional demethylation of germline DNA is supported by analysis of other sequence features of these regions as well as by previously published data on the methylation status in sperm DNA of two of these CG-rich regions.

MeSH Terms
Animals Base Sequence Dinucleoside Phosphates Genes Major Histocompatibility Complex Methylation Mice Models, Genetic Oligonucleotides/analysis Oligoribonucleotides/analysis
Chemicals
Dinucleoside Phosphates Oligonucleotides Oligoribonucleotides cytidylyl-3'-5'-guanosine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tykocinski M L
Max E E
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22 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.10.4385
Published
1984-05-25
Pages
4385-96
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318839
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/12.10.4385 PMC Full Text View journal details
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