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PMID: 6592607 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Ca2+ causes active contraction of bile canaliculi: direct evidence from microinjection studies.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 19 ·1984-10-00 ·Pages 6164-8

Watanabe S, Phillips MJ

Abstract

Cytoplasmic microinjection of Ca2+ triggers contraction of bile canaliculi in freshly isolated monolayer cultures of rat hepatocytes. Unseparated paired hepatocytes were used to demonstrate this motility-based phenomenon. Only one cell of the pair was injected, but fluorescein spread from the target cell to the opposite cell; also, the contractions were always uniform, equally involving both hepatocytes that form the canaliculus, indicating that communication exists between the cell pairs. Inhibitors of calmodulin and actin filaments, trifluoperazine and cytochalasin B, respectively, inhibited the Ca2+-induced contractions. Hence, the mechanism of contraction has features in common with actin-myosin based cytoplasmic motility behavior found in other non-muscle cells.

MeSH Terms
Animals Calcium/administration & dosage,pharmacology Cell Communication/drug effects Cell Movement/drug effects Cells, Cultured Cytochalasin B/pharmacology Female Gallbladder/physiology Liver/drug effects,physiology Microinjections Rats Rats, Inbred Strains Trifluoperazine/pharmacology
Chemicals
Trifluoperazine Cytochalasin B Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Watanabe S
Phillips M J
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34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.19.6164
Published
1984-10-00
Pages
6164-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391880
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.81.19.6164 PMC Full Text View journal details
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