Transfer from Rhizobium japonicum to Azotobacter vinelandii of genes required for nodulation.

Journal of bacteriology ·Vol. 134 ·No. 3 ·1978-06-00 ·Pages 1199-201

Maier RJ, Bishop PE, Brill WJ

Abstract

A mutant strain of Azotobacter vinelandii that is unable to fix N2 (Nif-) was transformed to Nif+ with DNA from Rhizobium japonicum. Of 50 Nif+ transformants tested, 3 contained the O antigen-related polysaccharide that is present on the cell surface of a nodulating R. japonicum strain, but is absent from a non-nodulating mutant strain.

MeSH Terms

Agglutination Tests Antigens, Bacterial/analysis Azotobacter/genetics,immunology,metabolism Genes Mutation Nitrogen Fixation Polysaccharides, Bacterial/analysis Rhizobium/genetics,immunology Transformation, Bacterial

Chemicals

Antigens, Bacterial Polysaccharides, Bacterial

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Maier R J

Bishop P E

Brill W J

References (10)

10 references, click to expand

Physiological factors affecting transformation of Azotobacter vinelandii.
J Bacteriol. 1976 Mar;125(3):1080-7 PMID: 3492
Cross-reactive antigens and lectin as determinants of symbiotic specificity in the Rhizobium-clover association.
Appl Microbiol. 1975 Dec;30(6):1017-33 PMID: 55100
Involvement of Rhizobium japonicum O antigen in soybean nodulation.
J Bacteriol. 1978 Mar;133(3):1295-9 PMID: 565351
Intergeneric transfer of genes involved in the Rhizobium-legume symbiosis.
Science. 1977 Dec 2;198(4320):938-40 PMID: 929179
Lectins: a possible basis for specificity in the Rhizobium--legume root nodule symbiosis.
Science. 1974 Jul 19;185(4147):269-71 PMID: 17812054
Ineffective and non-nodulating mutant strains of Rhizobium japonicum.
J Bacteriol. 1976 Aug;127(2):763-9 PMID: 986388
Nitrogenase. 3. Nitrogenaseless mutants of Azotobacter vinelandii: activities, cross-reactions and EPR spectra.
Biochim Biophys Acta. 1973 Jan 18;292(1):246-55 PMID: 4350172
Formation of the nitrogen-fixing enzyme system in Azotobacter vinelandii.
Can J Microbiol. 1968 Jan;14(1):25-31 PMID: 5644401
Receptor site on clover and alfalfa roots for Rhizobium.
Appl Environ Microbiol. 1977 Jan;33(1):132-6 PMID: 16345183
Host-symbiont interactions. I. The lectins of legumes interact with the o-antigen-containing lipopolysaccharides of their symbiont Rhizobia.
Biochem Biophys Res Commun. 1976 Jun 7;70(3):729-37 PMID: 945740

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.134.3.1199-1201.1978

Published

1978-06-00

Pages

1199-201

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC222375

Subset

External Links

PubMed source DOI: 10.1128/jb.134.3.1199-1201.1978 PMC Full Text View journal details

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Transfer from Rhizobium japonicum to Azotobacter vinelandii of genes required for nodulation.

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