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PMID: 6594708 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Immunohistochemical evidence for the coexistence of histidine decarboxylase-like and glutamate decarboxylase-like immunoreactivities in nerve cells of the magnocellular nucleus of the posterior hypothalamus of rats.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 23 ·1984-12-00 ·Pages 7647-50

Takeda N, Inagaki S, Shiosaka S, Taguchi Y, Oertel WH, Tohyama M, Watanabe T, Wada H

Abstract

Immunohistochemical staining of alternate consecutive sections revealed numerous histidine decarboxylase (L-histidine carboxy-lyase, EC 4.1.1.22)-like immunoreactive neurons that also contained glutamate decarboxylase (L-glutamate 1-carboxy-lyase, EC 4.1.1.15)-like immunoreactive structures in the tuberal magnocellular nucleus, the caudal magnocellular nucleus, and the postmammillary caudal magnocellular nucleus of the posterior hypothalamus of rats. Furthermore, in immunohistochemical double-staining procedures, almost all neurons in the magnocellular nuclei had both histidine decarboxylase-like and glutamate decarboxylase-like immunoreactivities. These results suggest the coexistence of histamine and gamma-aminobutyric acid in single neurons in these nuclei.

MeSH Terms

Animals Antibodies Antigen-Antibody Complex Carboxy-Lyases/analysis Glutamate Decarboxylase/analysis Histidine Decarboxylase/analysis Histocytochemistry Hypothalamus/enzymology Hypothalamus, Posterior/cytology,enzymology Liver/enzymology Male Neurons/enzymology Rats Rats, Inbred Strains

Chemicals

Antibodies Antigen-Antibody Complex Carboxy-Lyases Glutamate Decarboxylase Histidine Decarboxylase

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Takeda N

Inagaki S

Shiosaka S

Taguchi Y

Oertel W H

Tohyama M

Watanabe T

Wada H

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.81.23.7647

Published

1984-12-00

Pages

7647-50

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC392205

Subset

External Links

PubMed source DOI: 10.1073/pnas.81.23.7647 PMC Full Text View journal details

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Immunohistochemical evidence for the coexistence of histidine decarboxylase-like and glutamate decarboxylase-like immunoreactivities in nerve cells of the magnocellular nucleus of the posterior hypothalamus of rats.

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