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PMID: 6600269 Published · ppublish English Journal Article

Differentiation antigens identify subpopulations of rabbit T and B lymphocytes. Definition by flow cytometry.

The Journal of experimental medicine ·Vol. 157 ·No. 1 ·1983-01-01 ·Pages 34-46

Jackson S, Chused TM, Wilkinson JM, Leiserson WM, Kindt TJ

Abstract

A panel of six monoclonal antibodies produced against cell surface glycoproteins of a rabbit T lymphocyte line was used with flow cytometry to define rabbit lymphocyte subpopulations. Four thymocyte populations were characterized by size and expression of cell surface antigens and appear to represent stages in thymocyte differentiation. Rabbit spleen contained five subpopulations: two of T lineage, two of B, and a null cell subset. Bimodal distribution of staining of thymocytes and peripheral T cells was observed using an antibody (9AE10) directed against a Thy-1 analogue in the rabbit, suggesting two separate T cell lineages. One of the monoclonal reagents, L11/135, reacted strongly with peripheral rabbit T cells as shown by two-color immunofluorescence. In functional studies, only the L11/135-bearing cells responded to the T cell mitogens concanavalin A and phytohemagglutinin and to allogeneic splenocytes. The thymocyte subpopulations and the peripheral T and B cell subsets differ from those described in mouse and man.

MeSH Terms
Animals Antigens, Surface/analysis B-Lymphocytes/cytology,immunology Cell Differentiation Flow Cytometry Lymphocytes/classification Lymphocytes, Null/immunology Rabbits Spleen/immunology T-Lymphocytes/cytology,immunology Thymus Gland/immunology
Chemicals
Antigens, Surface
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Jackson S
Chused T M
Wilkinson J M
Leiserson W M
Kindt T J
References (20)
20 references, click to expand
  1. Surface Ig on rabbit lymphocytes. Rabbit B and T cells are distinct populations.
    Eur J Immunol. 1979 Dec;9(12):997-1003 PMID: 398775
  2. Biochemical analysis of human T lymphocyte differentiation antigens T4 and T5.
    Science. 1980 Jul 25;209(4455):520-1 PMID: 6967228
  3. Characterization of a human B lymphocyte-specific antigen.
    J Immunol. 1980 Oct;125(4):1678-85 PMID: 6157744
  4. Characterization of a human B cell-specific antigen (B2) distinct from B1.
    J Immunol. 1981 May;126(5):1941-7 PMID: 6163824
  5. Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man.
    J Exp Med. 1981 Feb 1;153(2):310-23 PMID: 6165796
  6. Functional studies of rabbit T lymphocytes.
    Immunology. 1981 Aug;43(4):635-41 PMID: 6456220
  7. Stages of B cell differentiation in human lymphoid tissue.
    J Exp Med. 1981 Sep 1;154(3):737-49 PMID: 7024458
  8. B lymphocyte subpopulation defined by a rat monoclonal antibody, 14G8.
    J Immunol. 1982 May;128(5):2049-56 PMID: 6801129
  9. Microdetermination of rabbit immunoglobulin allotypes by ELISA using specific antibodies conjugated with peroxidase or with biotin.
    J Immunol Methods. 1982;48(3):299-309 PMID: 7061867
  10. Monoclonal antibodies to rabbit lymphoid cells: preparation and characterization of a T-cell-specific antibody.
    Mol Immunol. 1981 Sep;18(9):815-22 PMID: 7040945
  11. Expression of Lyt-1 by a subset of B lymphocytes.
    J Immunol. 1982 Aug;129(2):532-8 PMID: 6177768
  12. In vitro demonstration of two antigenically-distinct rabbit lymphocyte populations.
    J Immunol. 1972 Aug;109(2):294-303 PMID: 5047371
  13. Fluorescent antibody staining. 3. Preparation of fluorescein-isothiocyanate-labeled antibodies.
    J Immunol. 1965 Aug;95(2):225-9 PMID: 4158327
  14. Clonal analysis of human cytotoxic T lymphocytes: T4+ and T8+ effector T cells recognize products of different major histocompatibility complex regions.
    Proc Natl Acad Sci U S A. 1982 Jul;79(14):4395-9 PMID: 6981813
  15. Cells involved in the immune response. XXII. The demonstration of thymus-specific antigens in the rabbit.
    Immunology. 1972 Nov;23(5):655-64 PMID: 4117948
  16. A membrane antigen of rabbit thymus cells.
    Cell Immunol. 1973 Jun;7(3):484-501 PMID: 4578140
  17. Isolation and characterization of a rabbit T lymphocyte-specific antigen.
    J Immunol. 1978 Apr;120(4):1429-35 PMID: 305942
  18. Separation of functional subsets of human T cells by a monoclonal antibody.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):4061-5 PMID: 315070
  19. The rabbit immune system: characterization of cell surface markers and functional properties of rabbit lymphocytes.
    Mol Immunol. 1979 Dec;16(12):1045-58 PMID: 161609
  20. T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views.
    J Exp Med. 1980 Aug 1;152(2):280-95 PMID: 6156984
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.157.1.34
Published
1983-01-01
Pages
34-46
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2186890
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.157.1.34 PMC Full Text View journal details
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