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PMID: 6603295 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

In vitro immunoglobulin synthesis by lymphocytes from patients with rheumatoid arthritis. I. Effect of monocyte depletion and demonstration of an increased proportion of lymphocytes forming rosettes with mouse erythrocytes.

Clinical and experimental immunology ·Vol. 52 ·No. 3 ·1983-06-00 ·Pages 505-11

Plater-Zyberk C, Clarke MF, Lam K, Mumford PA, Room GR, Maini RN

Abstract

We have investigated B cell function in nine patients with rheumatoid arthritis (RA) compared to sex and age matched controls in a pokeweed mitogen driven system. Levels of IgG and IgM synthesized in the supernatant were measured by a competition ELISA. We have found that cultured mononuclear cells from RA patients showed a defective Ig synthesis when depleted of monocytes. In contrast RA mononuclear cells not depleted of monocytes produced substantial levels of Ig after stimulation by the mitogen. The percentages of T and B lymphocytes in the peripheral blood of RA patients were normal; however, an increased number of lymphocytes formed rosettes with mouse erythrocytes indicating an abnormality in the B cell pool. These results demonstrate defective in vitro immunoglobulin synthesis by RA lymphocytes and show the importance of monocytes in this culture system.

MeSH Terms
Adult Aged Arthritis, Rheumatoid/immunology B-Lymphocytes/immunology Erythrocytes/immunology Female Humans Immunoglobulin G/biosynthesis Immunoglobulin M/biosynthesis Leukocyte Count Lymphocyte Activation Lymphocytes/immunology Male Middle Aged Monocytes/immunology Pokeweed Mitogens/pharmacology Rosette Formation
Chemicals
Immunoglobulin G Immunoglobulin M Pokeweed Mitogens
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Plater-Zyberk C
Clarke M F
Lam K
Mumford P A
Room G R
Maini R N
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30 references, click to expand
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Article Info
Journal
Clinical and experimental immunology
Abbr.
Clin Exp Immunol
ISSN
0009-9104
Published
1983-06-00
Pages
505-11
Language
English
Region
England
NLM ID
0057202
PMCID
PMC1536046
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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