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PMID: 6604698 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Further studies of the role of noncapsular antibody in protection against experimental Haemophilus influenzae type b bacteremia.

Infection and immunity ·Vol. 42 ·No. 1 ·1983-10-00 ·Pages 257-63

Shenep JL, Munson RS, Barenkamp SJ, Granoff DM

Abstract

Serum antibody against polyribosylribitol phosphate, the capsular antigen of Haemophilus influenzae type b, confers protection against experimental Haemophilus infection. Antibodies against noncapsular antigens are also protective, but the antigenic specificity of the protective antibodies remains unknown. Antilipopolysaccharide antibody was prepared by immunization of rabbits with boiled H. influenzae type b cells. Antilipopolysaccharide antibodies present in these sera did not protect against experimental Haemophilus bacteremia in infant rats. Antisera were also prepared by immunization of rabbits with live H. influenzae type b bacteria. After absorption of anticapsular and antilipopolysaccharide antibodies, these sera contained antibody to several outer membrane proteins which were accessible on the intact bacterial surface as detected by radioimmune precipitation. These absorbed sera prevented experimental Haemophilus bacteremia in infant rats. Thus, antibodies against noncapsular, non-lipopolysaccharide determinants, possibly against one or more outer membrane proteins, confer protection against experimental H. influenzae type b disease. In contrast, antibodies against lipopolysaccharide are ineffective.

MeSH Terms
Animals Antibodies, Bacterial/immunology Bacterial Outer Membrane Proteins Bacterial Proteins/immunology Haemophilus Infections/immunology Haemophilus influenzae/immunology Immunization, Passive Lipopolysaccharides/immunology Membrane Proteins/immunology Polysaccharides/immunology Polysaccharides, Bacterial/immunology Rabbits Rats Rats, Inbred Strains Sepsis/immunology
Chemicals
Antibodies, Bacterial Bacterial Outer Membrane Proteins Bacterial Proteins Lipopolysaccharides Membrane Proteins Polysaccharides Polysaccharides, Bacterial polyribitol phosphate
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Shenep J L
Munson R S
Barenkamp S J
Granoff D M
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28 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.42.1.257-263.1983
Published
1983-10-00
Pages
257-63
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC264552
Subset
IM
Grants
NIAID NIH HHS · 1-T32-AI 07172 · United States
NIAID NIH HHS · AI 17572 · United States
External Links
PubMed source DOI: 10.1128/iai.42.1.257-263.1983 PMC Full Text View journal details
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