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PMID: 6605975 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Enzyme-linked immunosorbent assay for detection of capsular antibodies against Haemophilus influenzae type b: comparison with radioimmunoassay.

Journal of clinical microbiology ·Vol. 18 ·No. 5 ·1983-11-00 ·Pages 1201-4

Kaplan SL, Mason EO, Johnson G, Broughton RA, Hurley D, Parke JC

Abstract

An enzyme-linked immunosorbent assay (ELISA) is described for detection of anti-capsular antibodies against Haemophilus influenzae type b. Polyribosephosphate was covalently bonded to poly-L-lysine before adsorption to microtiter plates. ELISA immunoglobulin G and immunoglobulin M anti-polyribosephosphate antibody titers were comparable to total anti-polyribosephosphate antibody concentration determined by radioimmunoassay. The ELISA technique will be useful for further investigations of host response to infections due to H. influenzae type b but is not intended to be used as a serological method for documenting H. influenzae type b infections.

MeSH Terms
Adsorption Antibodies, Bacterial/analysis Child Enzyme-Linked Immunosorbent Assay Haemophilus influenzae/immunology Humans Pentosephosphates/immunology Polysaccharides, Bacterial/immunology Radioimmunoassay
Chemicals
Antibodies, Bacterial Pentosephosphates Polysaccharides, Bacterial polyribose phosphate
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Kaplan S L
Mason E O
Johnson G
Broughton R A
Hurley D
Parke J C
References (12)
12 references, click to expand
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.18.5.1201-1204.1983
Published
1983-11-00
Pages
1201-4
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC272867
Subset
IM
Grants
NINDS NIH HHS · R01-NS-19292-04/04 · United States
External Links
PubMed source DOI: 10.1128/jcm.18.5.1201-1204.1983 PMC Full Text View journal details
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