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PMID: 6607033 Published · ppublish English Journal Article

Properties of novel beta-lactamase produced by Bacteroides fragilis.

Antimicrobial agents and chemotherapy ·Vol. 24 ·No. 6 ·1983-12-00 ·Pages 925-9

Yotsuji A, Minami S, Inoue M, Mitsuhashi S

Abstract

Bacteroides fragilis strains were isolated from clinical specimens. B. fragilis G-237 was highly resistant to beta-lactam antibiotics due to beta-lactamase production. The purified enzyme from this strain gave a single protein band on polyacrylamide gel electrophoresis. The isoelectric point was 4.8, and the molecular weight was estimated to be 26,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The enzyme activity was inhibited by p-chloromercuribenzoate and iodine but not by clavulanic acid or sulbactam. The purified enzyme showed a unique substrate profile by hydrolyzing at a high rate most of the cephalosporins, including cephamycin derivatives, penicillins, and imipenem (formerly imipemide, N-formimidoyl thienamycin, or MK 0787).

MeSH Terms
Bacteroides fragilis/enzymology Chemical Phenomena Chemistry Isoelectric Focusing Kinetics Microbial Sensitivity Tests Molecular Weight Moxalactam/metabolism beta-Lactamase Inhibitors beta-Lactamases/isolation & purification
Chemicals
beta-Lactamase Inhibitors beta-Lactamases Moxalactam
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Yotsuji A
Minami S
Inoue M
Mitsuhashi S
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20 references, click to expand
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.24.6.925
Published
1983-12-00
Pages
925-9
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC185409
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.24.6.925 PMC Full Text View journal details
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