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PMID: 6607875 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Mitogenicity of Naegleria fowleri extract for murine T lymphocytes.

Immunology ·Vol. 51 ·No. 3 ·1984-03-00 ·Pages 461-8

Ferrante A, Smyth C

Abstract

The whole-killed pathogenic free-living amoeba, Naegleria fowleri, contained mitogenic activity (NFM) for mouse spleen cells. Similar preparations from the non-pathogenic amoeba N. gruberi and the pathogenic Acanthamoeba culbertsoni lacked mitogenic activity. Fluids from N. fowleri cultures, containing amoeba antigens, also failed to cause proliferation of mouse spleen lymphocytes. Spleen cells from athymic nude mice failed to respond to NFM. In addition, nylon wool non-adherent, but not the adherent, spleen cell subpopulation proliferated in the presence of NFM. These results show that the factor(s) is mitogenic for T lymphocytes. The spleen cells from mice treated with cyclophosphamide doses known to deplete T suppressor cell activity from this organ failed to respond to NFM, indicating that NFM may be mitogenic for T suppressor cells.

MeSH Terms
Amoeba/immunology Animals B-Lymphocytes/immunology Cell Adhesion Cells, Cultured Cyclophosphamide/pharmacology Dose-Response Relationship, Immunologic Leukocyte Count Mice Mice, Inbred BALB C Mice, Nude Mitogens/pharmacology Mitosis Spleen/immunology T-Lymphocytes/immunology
Chemicals
Mitogens Cyclophosphamide
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ferrante A
Smyth C
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24 references, click to expand
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Article Info
Journal
Immunology
Abbr.
Immunology
ISSN
0019-2805
Published
1984-03-00
Pages
461-8
Language
English
Region
England
NLM ID
0374672
PMCID
PMC1454447
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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