Home LiteratureArticle Details
PMID: 6615464 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Endocytosis of liposomes bound to cell surface proteins measured by flow cytofluorometry.

The Biochemical journal ·Vol. 214 ·No. 1 ·1983-07-15 ·Pages 189-94

Truneh A, Mishal Z, Barbet J, Machy P, Leserman LD

Abstract

A new technique for the quantification of cellular receptor-mediated endocytosis has been developed based on the analysis by flow cytometry of ligand-bearing liposomes containing the fluorochrome carboxyfluorescein. Carboxyfluorescein encapsulated at high concentrations in protein A-bearing liposomes is self-quenched. Binding and internalization of such liposomes by cells via antibodies directed towards membrane surface determinants results in the release of the liposome-encapsulated carboxyfluorescein into the cytoplasm causing an increase in cell-associated fluorescence. This increase can be quantified on a flow cytofluorometer.

MeSH Terms
Animals Antibodies/immunology Endocytosis Fibronectins/immunology Flow Cytometry/methods Fluoresceins/metabolism H-2 Antigens/immunology Liposomes/metabolism Membrane Proteins/metabolism Mice Mice, Inbred CBA Staphylococcal Protein A/metabolism Temperature
Chemicals
Antibodies Fibronectins Fluoresceins H-2 Antigens H-2K(K) antigen Liposomes Membrane Proteins Staphylococcal Protein A 6-carboxyfluorescein
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Truneh A
Mishal Z
Barbet J
Machy P
Leserman L D
References (24)
24 references, click to expand
  1. Coated pits, coated vesicles, and receptor-mediated endocytosis.
    Nature. 1979 Jun 21;279(5715):679-85 PMID: 221835
  2. Cell-type-specific markers for distinguishing and studying neurons and the major classes of glial cells in culture.
    Brain Res. 1979 Oct 5;174(2):283-308 PMID: 385109
  3. Fluorescently labelled molecules as probes of the structure and function of living cells.
    Nature. 1980 Apr 3;284(5755):405-10 PMID: 6987537
  4. Functional relationships between insulin binding, action, and degradation. A reassessment.
    J Biol Chem. 1980 Nov 10;255(21):10052-5 PMID: 7000769
  5. Receptor-mediated endocytosis of antibody-opsonized liposomes by tumor cells.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):4089-93 PMID: 7001454
  6. Targeting to cells of fluorescent liposomes covalently coupled with monoclonal antibody or protein A.
    Nature. 1980 Dec 11;288(5791):602-4 PMID: 7442804
  7. Kinetics of antibody-dependent binding of haptenated phospholipid vesicles to a macrophage-related cell line.
    Biochemistry. 1980 Nov 11;19(23):5376-86 PMID: 7448176
  8. Binding and internalization of 125I-alpha 2-macroglobulin by cultured fibroblasts.
    J Biol Chem. 1981 Apr 10;256(7):3454-9 PMID: 6162847
  9. Membrane recycling by coated vesicles.
    Annu Rev Biochem. 1981;50:85-101 PMID: 7023370
  10. Cell-specific drug transfer from liposomes bearing monoclonal antibodies.
    Nature. 1981 Sep 17-23;293(5829):226-8 PMID: 7278980
  11. Journey to the center of the cell: role of the receptosome.
    Science. 1981 Oct 30;214(4520):504-9 PMID: 6170111
  12. Dynamics of antibody- and lectin-mediated endocytosis of hapten-containing liposomes by murine macrophages.
    Eur J Immunol. 1981 Sep;11(9):710-6 PMID: 7197629
  13. A surface marker for murine vascular endothelial cells defined by monoclonal antibody.
    J Histochem Cytochem. 1982 Feb;30(2):165-70 PMID: 7061819
  14. Kinetics of internalization and recycling of the asialoglycoprotein receptor in a hepatoma cell line.
    J Biol Chem. 1982 Apr 25;257(8):4230-7 PMID: 6279629
  15. Surface binding and rates of internalization of 125I-insulin in adipocytes and IM-9 lymphocytes.
    J Biol Chem. 1982 Aug 10;257(15):8667-73 PMID: 7047524
  16. Differential endocytosis of T and B lymphocyte surface molecules evaluated with antibody-bearing fluorescent liposomes containing methotrexate.
    Proc Natl Acad Sci U S A. 1982 Jul;79(13):4148-52 PMID: 7051006
  17. Drug transfer into lymphoblasts mediated by liposomes bound to distinct sites on H-2 encoded I-A, I-E, and K molecules.
    J Immunol. 1982 Nov;129(5):2098-102 PMID: 6811657
  18. Fibronectins: multifunctional modular glycoproteins.
    J Cell Biol. 1982 Nov;95(2 Pt 1):369-77 PMID: 6128348
  19. A rapid method for the isolation of functional thymus-derived murine lymphocytes.
    Eur J Immunol. 1973 Oct;3(10):645-9 PMID: 4587740
  20. Fluorescein: a complete antigenic group.
    Immunochemistry. 1976 May;13(5):447-53 PMID: 939585
  21. Liposome-cell interaction: transfer and intracellular release of a trapped fluorescent marker.
    Science. 1977 Feb 4;195(4277):489-92 PMID: 835007
  22. Liposome--lymphocyte interaction: saturable sites for transfer and intracellular release of liposome contents.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5603-7 PMID: 271988
  23. Antibody-mediated targeting of liposomes. Binding to lymphocytes does not ensure incorporation of vesicle contents into the cells.
    Biochim Biophys Acta. 1978 May 18;509(2):272-88 PMID: 656414
  24. Fluorescence probe measurement of the intralysosomal pH in living cells and the perturbation of pH by various agents.
    Proc Natl Acad Sci U S A. 1978 Jul;75(7):3327-31 PMID: 28524
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2140189
Published
1983-07-15
Pages
189-94
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1152225
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2140189 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2