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PMID: 661748 Published · ppublish English Journal Article

Determination of IgG, IgM, and IgA antibodies to Mycoplasma pneumoniae by an indirect staphylococcal radioimmunoassy.

Medical microbiology and immunology ·Vol. 165 ·No. 1 ·1978-05-26 ·Pages 29-41

Brunner H, Schaeg W, Brück U, Schummer U, Sziegoleit D, Schiefer H

Abstract

An indirect staphylococcal radioimmunoassay (SRIA) has been developed for determination of M. pneumoniae antibodies. This test allows the detection of antibodies in various immunoglobulin (Ig) classes similar to the previously described radioimmunoprecipitation test (RIP). SRIA has two advantages over RIP: first, it uses 100-fold less anti-Ig reagents than RIP; second, bound can be separated from unbound antigen more easily by the relatively heavy staphylococci. SRIA antibodies, belonging to the IgA class of Ig, could be detected in nasal secretions of volunteers infected intranasally with ts H43 of M. pneumoniae. In sera of patients with M. pneumoniae pneumonia antibodies to the IgG or the IgM class of Igs could be determined separately. This is especially important for an early diagnosis of M. pneumoniae disease.

MeSH Terms
Antibodies, Bacterial/analysis Humans Immunoglobulin A/analysis Immunoglobulin G/analysis Immunoglobulin M/analysis Mucus/immunology Mycoplasma/immunology Nasal Mucosa/metabolism Radioimmunoassay/methods Sputum/immunology Staphylococcal Protein A/immunology Staphylococcus aureus/immunology
Chemicals
Antibodies, Bacterial Immunoglobulin A Immunoglobulin G Immunoglobulin M Staphylococcal Protein A
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Brunner H
Schaeg W
Brück U
Schummer U
Sziegoleit D
Schiefer H
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28 references, click to expand
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Article Info
Journal
Medical microbiology and immunology
Abbr.
Med Microbiol Immunol
ISSN
0300-8584
DOI
10.1007/BF02121230
Published
1978-05-26
Pages
29-41
Language
English
Region
Germany
NLM ID
0314524
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02121230 View journal details
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