Home LiteratureArticle Details
PMID: 6618667 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Identification and characterization of Campylobacter jejuni outer membrane proteins.

Infection and immunity ·Vol. 42 ·No. 1 ·1983-10-00 ·Pages 276-84

Blaser MJ, Hopkins JA, Berka RM, Vasil ML, Wang WL

Abstract

Outer membrane proteins from isolates of Campylobacter jejuni were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Sarcosinate-insoluble membrane preparations were outer membrane enriched based on increased ketodeoxyoctonate concentrations, the presence of surface-exposed 125I-labeled proteins that were hydrophobic, and similarity to membrane vesicle (bleb) sodium dodecyl sulfate-polyacrylamide gel electrophoresis profiles. Most isolates contained a single major band with molecular weight of 41,000 to 45,000. Profiles of C. jejuni and Campylobacter coli isolates were indistinguishable, but either could be easily differentiated from Campylobacter fetus and Campylobacter faecalis. The profiles were stable for strains under a variety of growth, incubation and passage conditions. We classified 110 isolates from patients with sporadic campylobacter enteritis into nine subtypes based on differences in outer membrane sodium dodecyl sulfate-polyacrylamide gel electrophoresis profiles. Two categories accounted for 76% of the isolates. Complete concordance was observed in subtypes of strains obtained from epidemiologically related cases. Thus, comparison of the major outer membrane proteins of C. jejuni is a useful technique for investigating the transmission of this organism and may provide a basis for immunological characterization of the outer membrane proteins.

MeSH Terms
Bacterial Outer Membrane Proteins Bacterial Proteins/analysis Campylobacter/analysis Campylobacter fetus/analysis,classification Electrophoresis, Polyacrylamide Gel Membrane Proteins/analysis Molecular Weight Species Specificity Sugar Acids/analysis
Chemicals
Bacterial Outer Membrane Proteins Bacterial Proteins Membrane Proteins Sugar Acids 2-keto-3-deoxyoctonate
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Blaser M J
Hopkins J A
Berka R M
Vasil M L
Wang W L
References (30)
30 references, click to expand
  1. Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane.
    J Biol Chem. 1972 Jun 25;247(12):3962-72 PMID: 4555955
  2. Solubilization of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium-lauryl sarcosinate.
    J Bacteriol. 1973 Sep;115(3):717-22 PMID: 4580564
  3. Release of endotoxin in the form of cell wall blebs during in vitro growth of Neisseria meningitidis.
    J Exp Med. 1973 Nov 1;138(5):1156-67 PMID: 4200775
  4. Resolution of bacterial proteins by polyacrylamide gel electrophoresis on slabs. Membrane, soluble, and periplasmic fractions.
    J Biol Chem. 1974 Jan 25;249(2):634-44 PMID: 4129205
  5. Epidemiologic studies of serotype antigens common to groups B and C Neisseria meningitidis.
    J Infect Dis. 1975 Mar;131(3):286-90 PMID: 805184
  6. Partial purification and properties of somatic antigen spontaneously released from Vibrio cholerae.
    Infect Immun. 1975 Jul;12(1):187-92 PMID: 806532
  7. Major outer membrane protein of Campylobacter fetus: physical and immunological characterization.
    Infect Immun. 1976 Apr;13(4):1258-65 PMID: 819374
  8. Influence of osmolarity of the growth medium on the outer membrane protein pattern of Escherichia coli.
    J Bacteriol. 1977 Aug;131(2):623-30 PMID: 328490
  9. Isolation and characterization of outer and inner membranes from Pseudomonas aeruginosa and effect of EDTA on the membranes.
    J Biochem. 1978 Jan;83(1):171-81 PMID: 415055
  10. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples.
    Anal Biochem. 1978 Jun 15;87(1):206-10 PMID: 98070
  11. The outer membrane proteins of Gram-negative bacteria: biosynthesis, assembly, and functions.
    Annu Rev Biochem. 1978;47:481-532 PMID: 354502
  12. Separation and characterization of the outer membrane of Pseudomonas aeruginosa.
    J Biochem. 1978 Jul;84(1):179-91 PMID: 99443
  13. Toxin A-deficient mutants of Pseudomonas aeruginosa PA103: isolation and characterization.
    Infect Immun. 1980 Jun;28(3):899-908 PMID: 6772570
  14. Size heterogeneity of Salmonella typhimurium lipopolysaccharides in outer membranes and culture supernatant membrane fragments.
    J Bacteriol. 1980 Nov;144(2):630-40 PMID: 7000751
  15. A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels.
    Anal Biochem. 1980 Jul 1;105(2):361-3 PMID: 6161559
  16. Identification and preliminary characterization of Vibrio cholerae outer membrane proteins.
    J Bacteriol. 1981 Feb;145(2):1018-24 PMID: 7462157
  17. Outer membrane protein composition in disease isolates of Haemophilus influenzae: pathogenic and epidemiological implications.
    Infect Immun. 1980 Dec;30(3):709-17 PMID: 6971807
  18. Subtyping isolates of Haemophilus influenzae type b by outer-membrane protein profiles.
    J Infect Dis. 1981 May;143(5):668-76 PMID: 6972422
  19. Identification of immunogenic outer membrane proteins of Haemophilus influenzae type b in the infant rat model system.
    Infect Immun. 1981 Jun;32(3):1084-92 PMID: 6972915
  20. Antigen-specific serotyping of Neisseria gonorrhoeae: characterization based upon principal outer membrane protein.
    Infect Immun. 1981 Jun;32(3):985-94 PMID: 6166568
  21. Campylobacter enteritis.
    N Engl J Med. 1981 Dec 10;305(24):1444-52 PMID: 7029281
  22. Passive hemagglutination technique for serotyping Campylobacter fetus subsp. jejuni on the basis of soluble heat-stable antigens.
    J Clin Microbiol. 1980 Dec;12(6):732-7 PMID: 6796598
  23. Surface-exposed protein antigens of the gonococcal outer membrane.
    Infect Immun. 1981 Dec;34(3):804-16 PMID: 6800957
  24. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels.
    Anal Biochem. 1982 Jan 1;119(1):115-9 PMID: 6176137
  25. Campylobacter enteritis in Denver.
    West J Med. 1982 Apr;136(4):287-90 PMID: 7090379
  26. 30 years of campylobacters: biochemical characteristics and a biotyping proposal for Campylobacter jejuni.
    J Clin Microbiol. 1982 Jun;15(6):1065-73 PMID: 7107840
  27. Sodium dodecyl sulfate-polyacrylamide gel typing system for characterization of Neisseria meningitidis isolates.
    J Clin Microbiol. 1982 Aug;16(2):240-4 PMID: 6811609
  28. Diversity of serotypes in outbreaks of enteritis due to Campylobacter jejuni.
    J Infect Dis. 1982 Dec;146(6):826 PMID: 7142751
  29. Campylobacter enteritis associated with foodborne transmission.
    Am J Epidemiol. 1982 Dec;116(6):886-94 PMID: 7148815
  30. Outer membrane characteristics of Campylobacter jejuni.
    Infect Immun. 1982 Dec;38(3):898-906 PMID: 7152677
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.42.1.276-284.1983
Published
1983-10-00
Pages
276-84
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC264555
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.42.1.276-284.1983 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2