RNA polymerase from Rhizobium japonicum.
DNA-dependent RNA polymerase (EC 2.7.7.6) from Rhizobium japonicum was purified. The subunit structure was found to be beta beta' alpha 2 alpha, with the following apparent molecular weights determined by electrophoresis: Mr (beta and beta') 150,000 each, Mr (sigma) 96,000, Mr (alpha) 40,000, Mr (holoenzyme) 490,000, Mr (core enzyme) 380,000. The recovery of sigma was 28%. RNA polymerase from aerobically grown R. japonicum cells and from nitrogen-fixing cells have the same electrophoretic properties suggesting that no chemical modification of the enzyme occurs when cells undergo this metabolic differentiation. The enzyme is Mg2+-dependent, rifampicin-sensitive, and has optimal activity at alkaline pH (8--10) and at 35--40 degrees C. It binds strongly to bacteriophage T7 promoters, weakly to antibiotic resistance genes, and not at all to cloned R. japonicum nif DNA. Preliminary in vitro transcription experiments, including nif DNA as template, revealed that additional factors may be required for selective transcription from promoters.
26 references, click to expand
-
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
-
The nucleoside triphosphate-ribonucleic acid nucleotidyltransferase (EC 2.7.7.6) of Agrobacterium tumefaciens (Smith and Townsend) Conn. Purification and properties of the enzyme from the tumorigenic strain B6806.Biochem J. 1974 Dec;143(3):511-20 PMID: 4462737
-
A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase.J Biol Chem. 1969 Nov 25;244(22):6160-7 PMID: 4900510
-
Variables affecting the selectivity and efficiency of retention of DNA fragments by E. coli RNA polymerase in the nitrocellulose-filter-binding assay.Gene. 1981 Jan-Feb;13(1):75-87 PMID: 7016668
-
Transcription in lactobacillaceae. DNA-dependent RNA polymerase from Lactobacillus curvatus.Eur J Biochem. 1974 Oct 2;48(2):527-40 PMID: 4614979
-
AN APPARATUS FOR PREPARATIVE TEMPERATURE-REGULATED POLYACRYLAMIDE GEL ELECTROPHORESIS.Anal Biochem. 1964 Nov;9:351-69 PMID: 14238529
-
The biosynthesis of nitrogenase MoFe protein polypeptides in free-living cultures of Rhizobium japonicum.Biochim Biophys Acta. 1979 Dec 17;565(2):365-78 PMID: 518887
-
Symbiotic effectiveness of antibiotic-resistant mutants of fast- and slow-growing strains of Rhizobium nodulating Lotus species.Can J Microbiol. 1977 Aug;23(8):1026-33 PMID: 890601
-
Primary structure of alpha-subunit of DNA-dependent RNA polymerase from Escherichia coli.FEBS Lett. 1977 Apr 1;76(1):108-11 PMID: 323055
-
Utilization of promoter and terminator sites on bacteriophage T7 DNA by RNA polymerases from a variety of bacterial orders.Cell. 1979 Jan;16(1):97-109 PMID: 421272
-
The primary structure of Escherichia coli RNA polymerase. Nucleotide sequence of the rpoB gene and amino-acid sequence of the beta-subunit.Eur J Biochem. 1981 Jun 1;116(3):621-9 PMID: 6266829
-
Mapping and characterization of promoters in bacteriophages fd, f1 and m13.J Mol Biol. 1975 Feb 25;92(2):261-77 PMID: 1095754
-
Escherichia coli RNA polymerase binding and initiation of transcription on fragments of lambda rifd 18 DNA containing promoters for lambda genes and for rrnB, tufB, rplC,A, rplJ,L, and rpoB,C genes.Gene. 1979 Aug;6(4):331-65 PMID: 159206
-
Nitrocellulose filter binding studies of the interactions of Escherichia coli RNA polymerase holoenzyme with deoxyribonucleic acid restriction fragments: evidence for multiple classes of nonpromoter interactions, some of which display promoter-like properties.Biochemistry. 1982 Aug 31;21(18):4318-31 PMID: 6289878
-
Purification and properties of the sigma subunit of Escherichia coli DNA-dependent RNA polymerase.Biochemistry. 1979 Apr 3;18(7):1344-52 PMID: 371677
-
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.Gene. 1977;2(2):95-113 PMID: 344137
-
Separation and characterization of the subunits of ribonucleic acid polymerase.J Biol Chem. 1969 Nov 25;244(22):6168-76 PMID: 4900511
-
DNA-dependent RNA polymerase from the archaebacterium Sulfolobus acidocaldarius.Eur J Biochem. 1979 Jun 1;96(3):597-604 PMID: 380989
-
Protein measurement with the Folin phenol reagent.J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
-
High resolution two-dimensional electrophoresis of proteins.J Biol Chem. 1975 May 25;250(10):4007-21 PMID: 236308
-
Species specificity of promoter recognition by RNA polymerase and its transfer by the sigma factor.Eur J Biochem. 1982 Jun;124(3):427-33 PMID: 6809459
-
Sequencing end-labeled DNA with base-specific chemical cleavages.Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
-
[Primary structure of Escherichia coli RNA-polymerase. The nucleotide sequence of the rpoC gene and the amino acid sequence of the beta'-subunit].Dokl Akad Nauk SSSR. 1981;261(3):763-8 PMID: 7032868
-
Nucleotide sequence from the genetic left end of bacteriophage T7 DNA to the beginning of gene 4.J Mol Biol. 1981 Jun 5;148(4):303-30 PMID: 7310871
-
Analytical and preparative electrophoresis of RNA in agarose-urea.Anal Biochem. 1979 Oct 1;98(2):358-67 PMID: 386836
-
Differentiation of Rhizobium japonicum, III. Inhibition of nitrogenase derepression by chloramphenicol and rifampicin concentrations, not inhibiting growth.Z Naturforsch C. 1978 Nov-Dec;33(11-12):859-62 PMID: 154223
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