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PMID: 6683707 Published · ppublish English Journal Article

Frequency of fragile X chromosomes, fra(X), in lymphocytes in relation to blood storage time and culture techniques.

Human genetics ·Vol. 64 ·No. 1 ·1983-00-00 ·Pages 39-41

Fonatsch C, Schwinger E

Abstract

The fra(X) frequencies in metaphases of lymphocytes from seven male patients with X-linked mental retardation and macroorchidism were scored after use of different culture techniques and different times of blood storage. No statistically significant differences were found between the fra(X) rates of lymphocytes grown either in folic acid deficient medium (TC 199) or in medium containing folic acid as well as a folic acid antagonist (methotrexate: MTX, aminopterine: AP). With respect to the effect of the time interval between blood sampling and culture set-up, a statistically significant decrease in the fra(X) frequency was observed in all culture types after 4 or 7 days of blood storage.

MeSH Terms
Blood Preservation Cells, Cultured Chromosome Fragility Female Gene Frequency Humans Intellectual Disability/genetics Lymphocytes/ultrastructure Male Metaphase Sex Chromosomes/ultrastructure Testis/abnormalities Time Factors X Chromosome/ultrastructure
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Fonatsch C
Schwinger E
References (22)
22 references, click to expand
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Article Info
Journal
Human genetics
Abbr.
Hum Genet
ISSN
0340-6717
DOI
10.1007/BF00289476
Published
1983-00-00
Pages
39-41
Language
English
Region
Germany
NLM ID
7613873
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00289476 View journal details
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