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PMID: 6690426 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cloning of genes involved in the biosynthesis of pseudobactin, a high-affinity iron transport agent of a plant growth-promoting Pseudomonas strain.

Journal of bacteriology ·Vol. 157 ·No. 1 ·1984-01-00 ·Pages 53-8

Moores JC, Magazin M, Ditta GS, Leong J

Abstract

A gene bank of DNA from plant growth-promoting Pseudomonas sp. strain B10 was constructed using the broad host-range conjugative cosmid pLAFR1. The recombinant cosmids contained insert DNA averaging 21.5 kilobase pairs in length. Nonfluorescent mutants of Pseudomonas sp. strain B10 were obtained by mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine, ethyl methanesulfonate, or UV light and were defective in the biosynthesis of its yellow-green, fluorescent siderophore (microbial iron transport agent) pseudobactin. No yellow-green, fluorescent mutants defective in the production of pseudobactin were identified. Nonfluorescent mutants were individually complemented by mating the gene bank en masse and identifying fluorescent transconjugants. Eight recombinant cosmids were sufficient to complement 154 nonfluorescent mutants. The pattern of complementation suggests that a minimum of 12 genes arranged in four gene clusters is required for the biosynthesis of pseudobactin. This minimum number of genes seems reasonable considering the structural complexity of pseudobactin.

MeSH Terms
Cloning, Molecular/drug effects,radiation effects DNA, Bacterial/genetics,radiation effects Ethyl Methanesulfonate/pharmacology Genes, Bacterial/drug effects,radiation effects Genetic Complementation Test Ionophores Methylnitronitrosoguanidine/pharmacology Mutation Oligopeptides/biosynthesis,genetics Plasmids/drug effects,radiation effects Pseudomonas/drug effects,genetics,metabolism Ultraviolet Rays
Chemicals
DNA, Bacterial Ionophores Oligopeptides Methylnitronitrosoguanidine Ethyl Methanesulfonate pseudobactin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Moores J C
Magazin M
Ditta G S
Leong J
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27 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.157.1.53-58.1984
Published
1984-01-00
Pages
53-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215128
Subset
IM
Grants
NIADDK NIH HHS · AM07233 · United States
External Links
PubMed source DOI: 10.1128/jb.157.1.53-58.1984 PMC Full Text View journal details
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