Peptide mapping characterization of viral proteins generated in a cell-free coupled system for the transcription and translation of influenza virus mRNA.

Journal of virology ·Vol. 26 ·No. 3 ·1978-06-00 ·Pages 817-21

Content J, De Wit L, Horisberger MA

Abstract

In a coupled cell-free system for the transcription and translation of the influenza mRNA's, containing detergent-disrupted purified NWS influenza virion and a micrococcal nuclease-preincubated rabbit reticulocyte lysate, five unglycosylated viral proteins (NS1, M, NP, P1, and P3) were easily produced and isolated. Their identification was based on the electrophoretic separation of peptide fragments resulting from their partial digestion with proteases of restricted specificity (D.W. Cleveland, S. G. Fisher, N. W. Kirschner, and U. K. Laemmli, J. Biol. Chem. 252:1102-1106, 1977).

MeSH Terms

Cell-Free System Orthomyxoviridae/metabolism Peptide Biosynthesis Protein Biosynthesis RNA, Messenger/metabolism RNA, Viral/metabolism Transcription, Genetic Viral Proteins/biosynthesis

Chemicals

RNA, Messenger RNA, Viral Viral Proteins

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Content J

De Wit L

Horisberger M A

References (14)

14 references, click to expand

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Suppression of glycoprotein formation of Semliki Forest, influenza, and avian sarcoma virus by tunicamycin.
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Polypeptides specified by the influenza virus genoma. 2. Assignement of protein coding functions to individual genome segments by in vitro translation.
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Cell-free coupling of influenza virus RNA transcription and translation.
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Cell-free translation of RNA synthesized in vitro by a transcribing nucleoprotein complex prepared from purified vesicular stomatitis virus.
J Virol. 1977 Mar;21(3):1002-9 PMID: 191633
Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.
J Biol Chem. 1977 Feb 10;252(3):1102-6 PMID: 320200
An efficient mRNA-dependent translation system from reticulocyte lysates.
Eur J Biochem. 1976 AUG 1;67(1):247-56 PMID: 823012
Early polypeptide synthesis in influenza virus-infected cells.
Virology. 1973 Nov;56(1):394-9 PMID: 4795674
Cell-free translation of influenza virus mRNA.
J Virol. 1976 May;18(2):604-18 PMID: 1271519
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FEBS Lett. 1976 Jun 15;65(3):365-8 PMID: 955071
Purification of influenza viral complementary RNA: its genetic content and activity in wheat germ cell-free extracts.
J Virol. 1975 Dec;16(6):1464-75 PMID: 1202245
Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
Eur J Biochem. 1975 Aug 15;56(2):335-41 PMID: 1175627

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.26.3.817-821.1978

Published

1978-06-00

Pages

817-21

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC525907

Subset

External Links

PubMed source DOI: 10.1128/JVI.26.3.817-821.1978 PMC Full Text View journal details

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Peptide mapping characterization of viral proteins generated in a cell-free coupled system for the transcription and translation of influenza virus mRNA.

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