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PMID: 6746910 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Molecular properties of the Ro/SSA antigen and enzyme-linked immunosorbent assay for quantitation of antibody.

The Journal of clinical investigation ·Vol. 74 ·No. 2 ·1984-08-00 ·Pages 625-33

Yamagata H, Harley JB, Reichlin M

Abstract

Antibodies to the Ro/SSA antigen occur in patients with systemic lupus erythematosus and Sjögren's syndrome. An immunoaffinity method for the preparation of electrophoretically homogeneous Ro/SSA antigen is described. Several molecular properties of the antigen have been determined. The native RNA protein particle has a molecular weight of approximately 100,000 D determined by gel filtration. Sodium dodecyl sulfate-analysis of the purified Ro/SSA antigen and analysis by staining of bands with silver and Coomassie Blue, Western blotting, and RNAase treatment leads to a hypothesis for the structure of the particle in which an antigenic 60,000 protein is bound to 24,000-27,000 RNA molecules which are not antigenic. An enzyme-linked immunoabsorbent assay method for assay of anti-Ro/SSA is also described which sensitively measures antigen binding at dilutions of sera containing anti-Ro/SSA precipitins up to 10(7) fold. Normal sera on average have 10(3) less binding activity.

MeSH Terms
Animals Antigen-Antibody Complex Antigens/immunology Autoantibodies/analysis Autoantigens Cattle Enzyme-Linked Immunosorbent Assay Humans Lupus Erythematosus, Systemic/immunology Lymphocytes/immunology Molecular Weight RNA, Small Cytoplasmic Reference Values Ribonucleoproteins Spleen/immunology Thymus Gland/immunology
Chemicals
Antigen-Antibody Complex Antigens Autoantibodies Autoantigens RNA, Small Cytoplasmic RO60 protein, human Ribonucleoproteins SS-A antigen
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Yamagata H
Harley J B
Reichlin M
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33 references, click to expand
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI111460
Published
1984-08-00
Pages
625-33
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC370515
Subset
IM
Grants
NIADDK NIH HHS · AM-31133 · United States
External Links
PubMed source DOI: 10.1172/JCI111460 PMC Full Text View journal details
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