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PMID: 6750320 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Separation of the minimal replication region of the F plasmid into a replication origin segment and a trans-acting segment.

Molecular & general genetics : MGG ·Vol. 186 ·No. 3 ·1982-00-00 ·Pages 372-7

Tolun A, Helinski DR

Abstract

An analysis was carried out on the replication functions within a 2.3 kilobase (kb) segment of the F plasmid which contains an origin (ori s) of replication and is capable of autonomous replication in Escherichia coli. Two separable regions were delineated for this segment: an origin region of approximately 1,140 bp in length and a segment of approximately 1,140 bp that functions in trans to support replication of the origin region. The trans-acting segment is functional as part of an F replicon or when inserted into the E. coli chromosome. A prominent feature of the trans-acting segment is a coding sequence for a 29 K protein (Murotsu et al. 1981).

MeSH Terms
DNA Replication DNA, Bacterial/biosynthesis,genetics Escherichia coli/genetics,metabolism Genes, Bacterial Plasmids Repetitive Sequences, Nucleic Acid
Chemicals
DNA, Bacterial
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tolun A
Helinski D R
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23 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00729456
Published
1982-00-00
Pages
372-7
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIAID NIH HHS · AI-07194 · United States
External Links
PubMed source DOI: 10.1007/BF00729456
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