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PMID: 6751259 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Metabolism of Saccharomyces cerevisiae envelope mannoproteins.

Archives of microbiology ·Vol. 132 ·No. 2 ·1982-08-00 ·Pages 144-8

Pastor FI, Herrero E, Sentandreu R

Abstract

By pulse and chase labeling experiments, two independent mannoprotein pools have been found associated with the Saccharomyces cerevisiae envelope. One of them probably corresponds to mannoproteins localized in the periplasmic space. These molecules showed a high turnover rate at 28 degrees C. The second pool is formed by intrinsic wall mannoproteins which are apparently stable for long periods of time, after a small initial turnover. These results suggest that at least part of the mannoproteins initially found in the periplasmic space may move into the wall. The time lag between the addition of the radioactive precursors and their incorporation in the cell envelope (20-30 min for amino acids and about 10 min for carbohydrate) indicates that protein formation and carbohydrate incorporation take place in succession. Moreover, bulk glycosylation of mannoproteins seems to occur close in time to the moment of secretion into the periplasmic space.

MeSH Terms
Cell Wall/metabolism Fungal Proteins/metabolism Glucan Endo-1,3-beta-D-Glucosidase/pharmacology Glycoproteins/metabolism Kinetics Mannans/metabolism Membrane Glycoproteins Saccharomyces cerevisiae/metabolism
Chemicals
Fungal Proteins Glycoproteins Mannans Membrane Glycoproteins mannoproteins Glucan Endo-1,3-beta-D-Glucosidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Pastor F I
Herrero E
Sentandreu R
References (23)
23 references, click to expand
  1. Characterization of two forms of asparaginase in Saccharomyces cerevisiae.
    J Biol Chem. 1978 Feb 25;253(4):1297-304 PMID: 342521
  2. Chitin and yeast budding. Localization of chitin in yeast bud scars.
    J Biol Chem. 1971 Jan 10;246(1):152-9 PMID: 5541754
  3. Cytochemical and ultrastructural studies of Candida albicans. II. Evidence for a cell wall coat using concanavalin A.
    J Ultrastruct Res. 1981 Apr;75(1):50-9 PMID: 7021871
  4. alpha-Galactosidase from Saccharomyces carlsbergensis. Cellular localization, and purification of the external enzyme.
    Eur J Biochem. 1977 Jul 15;77(2):375-82 PMID: 891541
  5. Isolation of glucanase-containing vesicles from budding yeast.
    Arch Mikrobiol. 1972;82(3):189-205 PMID: 4337552
  6. Synthesis of yeast wall glucan.
    J Gen Microbiol. 1975 Sep;90(1):13-20 PMID: 240909
  7. Biosynthesis of cell walls of fungi.
    Microbiol Rev. 1979 Jun;43(2):117-44 PMID: 390353
  8. Site of initial glycosylation of mannoproteins from Saccharomyces cerevisiae.
    J Bacteriol. 1975 Oct;124(1):127-33 PMID: 240803
  9. Two pools of glycogen in Saccharomyces.
    J Bacteriol. 1977 May;130(2):818-25 PMID: 45487
  10. Localization of acid phosphatase in Saccharomyces cerevisiae: a clue to cell wall formation.
    J Bacteriol. 1977 Aug;131(2):638-44 PMID: 328492
  11. Metabolic relationship between invertase and acid phosphatase isoenzymes in Saccharomyces cerevisiae.
    Biochim Biophys Acta. 1980 May 22;629(3):445-54 PMID: 6998502
  12. Timing and function of chitin synthesis in yeast.
    J Bacteriol. 1975 Dec;124(3):1586-93 PMID: 1104591
  13. Autoradiographic study of mannan incorporation into the growing cell walls of Saccharomyces cerevisiae.
    J Bacteriol. 1974 Jan;117(1):265-9 PMID: 4587607
  14. Effect of cycloheximide on yeast cell wall synthesis.
    Biochem Biophys Res Commun. 1969 Aug 22;36(5):741-7 PMID: 5808289
  15. The structure of the yeast cell wall. Solubilization of a marker enzyme, -fructofuranosidase, by the autolytic enzyme system.
    J Biol Chem. 1972 Feb 25;247(4):1161-9 PMID: 4551513
  16. Lipid-mediated glycosylation of endogenous proteins in isolated plasma membrane of Saccharomyces cerevisiae.
    J Bacteriol. 1980 Jan;141(1):342-9 PMID: 6986363
  17. Synthesis of 1,3-beta-glucanases in Saccharomyces cerevisiae during the mitotic cycle, mating, and sporulation.
    J Bacteriol. 1979 Sep;139(3):924-31 PMID: 113390
  18. Lysis of intact yeast cells and isolated cell walls by an inducible enzyme system of Arthrobacter GJM-1.
    Z Allg Mikrobiol. 1977;17(5):391-402 PMID: 22164
  19. Comparative study of the properties of the purified internal and external invertases from yeast.
    J Biol Chem. 1968 Apr 10;243(7):1573-7 PMID: 4967422
  20. Order of events in the yeast secretory pathway.
    Cell. 1981 Aug;25(2):461-9 PMID: 7026045
  21. Purification and properties of an enzyme, zymolyase, which lyses viable yeast cells.
    Arch Biochem Biophys. 1972 Nov;153(1):403-6 PMID: 4568261
  22. Wall mannan of Saccharomyces cerevisiae. Metabolic stability and release into growth medium.
    Biochim Biophys Acta. 1975 Sep 8;404(1):1-6 PMID: 1100119
  23. Structure and biosynthesis of the mannan component of the yeast cell envelope.
    Adv Microb Physiol. 1976;14(11):93-158 PMID: 795277
Article Info
Journal
Archives of microbiology
Abbr.
Arch Microbiol
ISSN
0302-8933
DOI
10.1007/BF00508720
Published
1982-08-00
Pages
144-8
Language
English
Region
Germany
NLM ID
0410427
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00508720 View journal details
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