A comparison of immunoferritin, immuno-enzyme and gold-labelled protein A methods for the localization of capsular antigen on frozen thin sections of the bacterium, Pasteurella haemolytica.

The Histochemical journal ·Vol. 14 ·No. 5 ·1982-09-00 ·Pages 803-10

Beesley JE, Orpin A, Adlam C

Abstract

The staphylococcal protein A-gold method was found to be superior to the enzyme-or ferritin-linked antibody techniques for locating capsular antigens on cryosections of Pasteurella haemolytica, and its sensitivity was similar to the enzyme-linked antibody method. The sensitivity of conventionally fixed and embedded material and cryosections of heavily fixed, lightly fixed and unfixed material were shown to be similar under routine laboratory conditions.

MeSH Terms

Antigens, Bacterial/analysis Colloids Ferritins Frozen Sections Gold Immunoenzyme Techniques Pasteurella/immunology Staphylococcal Protein A

Chemicals

Antigens, Bacterial Colloids Staphylococcal Protein A Gold Ferritins

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Beesley J E

Orpin A

Adlam C

References (12)

12 references, click to expand

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Article Info

Journal

The Histochemical journal

Abbr.

Histochem J

ISSN

0018-2214

DOI

10.1007/BF01033629

Published

1982-09-00

Pages

803-10

Language

English

Region

Netherlands

NLM ID

0163161

Subset

External Links

PubMed source DOI: 10.1007/BF01033629

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A comparison of immunoferritin, immuno-enzyme and gold-labelled protein A methods for the localization of capsular antigen on frozen thin sections of the bacterium, Pasteurella haemolytica.

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