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PMID: 6752202 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Association of hemoglobin C with erythrocyte ghosts.

The Journal of clinical investigation ·Vol. 70 ·No. 5 ·1982-11-00 ·Pages 946-52

Reiss GH, Ranney HM, Shaklai N

Abstract

The interaction of hemoglobin C (Hb C) with erythrocyte membranes was studied using changes in fluorescence intensity in a membrane-embedded probe. The affinity of Hb C for the membranes at pH 6.0 and pH 6.8 was compared to that of normal hemoglobin (Hb A). Steady-state and kinetic data were delivered. The affinity of Hb C for the erythrocyte membrane at pH 6.8 appeared to be about five times greater than that of Hb A. The associations of Hb C and Hb A with the membrane were reversible to about the same extent. The cytoplasmic portions of band 3 membrane proteins were suggested to be the binding sites for both hemoglobins. The membrane binding of Hb C at pH values of 6.8 to 7.0 indicates that this reaction may occur under physiological circumstances.

MeSH Terms
Binding Sites Erythrocyte Membrane/metabolism Erythrocytes/metabolism Fluorescent Antibody Technique Hemoglobin A/metabolism Hemoglobin C/metabolism Humans Hydrogen-Ion Concentration Kinetics Osmolar Concentration Oxyhemoglobins/metabolism,pharmacology
Chemicals
Oxyhemoglobins Hemoglobin C Hemoglobin A
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Reiss G H
Ranney H M
Shaklai N
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20 references, click to expand
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/jci110706
Published
1982-11-00
Pages
946-52
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC370307
Subset
IM
Grants
NIADDK NIH HHS · AM17348 · United States
NIADDK NIH HHS · AM18781 · United States
External Links
PubMed source DOI: 10.1172/jci110706 PMC Full Text View journal details
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