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PMID: 6762207 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The determination by radiochemical assay of argininosuccinase produced in an Escherichia coli system in vitro.

The Biochemical journal ·Vol. 207 ·No. 3 ·1982-12-01 ·Pages 529-33

Zidwick MJ, Rogers P

Abstract

A highly sensitive radiochemical assay used to measure the synthesis and regulation of the product of the argH gene, argininosuccinase, in an Escherichia coli system in vitro is described. With L-[guanidino-14C]argininosuccinic acid as a substrate, and in the presence of excess arginase and urease, 14CO2 is collected in a simply designed micro-vessel. With this method less than 1 nmol of product can be measured in the presence of various concentrations of L-arginine.

MeSH Terms

Argininosuccinate Lyase/biosynthesis Argininosuccinic Acid/metabolism Carbon Radioisotopes Escherichia coli/enzymology Lyases/biosynthesis Radiochemistry/methods

Chemicals

Carbon Radioisotopes Argininosuccinic Acid Lyases Argininosuccinate Lyase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Zidwick M J

Rogers P

References (16)

16 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2070529

Published

1982-12-01

Pages

529-33

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1153893

Subset

Grants

NIGMS NIH HHS · GM-25320 · United States

External Links

PubMed source DOI: 10.1042/bj2070529 PMC Full Text View journal details

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The determination by radiochemical assay of argininosuccinase produced in an Escherichia coli system in vitro.

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