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PMID: 6765200 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sequence and structure of yeast phosphoglycerate kinase.

The EMBO journal ·Vol. 1 ·No. 12 ·1982-00-00 ·Pages 1635-40

Watson HC, Walker NP, Shaw PJ, Bryant TN, Wendell PL, Fothergill LA, Perkins RE, Conroy SC, Dobson MJ, Tuite MF

Abstract

The structure of yeast phosphoglycerate kinase has been determined with data obtained from amino acid sequence, nucleotide sequence, and X-ray crystallographic studies. The substrate binding sites, as deduced from electron density maps, are compatible with known substrate specificity and the stereochemical requirements for the enzymic reaction. A carboxyl-imidazole interaction appears to be involved in controlling the transition between the open and closed forms of the enzyme.

MeSH Terms
Amino Acid Sequence Models, Molecular Phosphoglycerate Kinase/isolation & purification,metabolism Protein Conformation Saccharomyces cerevisiae/enzymology Substrate Specificity X-Ray Diffraction
Chemicals
Phosphoglycerate Kinase
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Watson H C
Walker N P
Shaw P J
Bryant T N
Wendell P L
Fothergill L A
Perkins R E
Conroy S C
Dobson M J
Tuite M F
References (22)
22 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1982-00-00
Pages
1635-40
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553262
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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