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PMID: 6765233 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Immunoglobulin V region variants in hybridoma cells. I. Isolation of a variant with altered idiotypic and antigen binding specificity.

The EMBO journal ·Vol. 1 ·No. 5 ·1982-00-00 ·Pages 629-34

Brüggemann M, Radbruch A, Rajewsky K

Abstract

The hybridoma cell line B1-8.delta 1 expresses an IgD antibody with specificity for the hapten 4-hydroxy-3-nitro-phenylacetyl (NP). Two monoclonal antibodies, each recognizing an idiotypic determinant (idiotope) in the variable region of antibody B1-8, and fluorescence-activated cell sorting were used for the isolation of spontaneous somatic cell variants whose antibody product lacks the expression of one of the two idiotopes. The variant cells carry a mutation which is expressed in the variable region of the antibody heavy chains and which reduces the affinity of the antibody for hapten greater than 100-fold. Idiotope loss variants of the selected phenotype are present in the B1-8.delta 1 cell population at low frequency.

MeSH Terms
Animals Antibodies, Monoclonal Antigen-Antibody Complex Cell Line Clone Cells Flow Cytometry Fluorescent Antibody Technique Genetic Variation Hybridomas/immunology Immunoglobulin Idiotypes/genetics Immunoglobulin Variable Region/genetics Mice Radioimmunoassay
Chemicals
Antibodies, Monoclonal Antigen-Antibody Complex Immunoglobulin Idiotypes Immunoglobulin Variable Region
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Brüggemann M
Radbruch A
Rajewsky K
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26 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1982-00-00
Pages
629-34
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553098
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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