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PMID: 6765595 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Characterization of a 40S ribosomal subunit complex in polyribosomes of Saccharomyces cerevisiae treated with cycloheximide.

Molecular and cellular biology ·Vol. 1 ·No. 1 ·1981-01-00 ·Pages 51-7

Helser TL, Baan RA, Dahlberg AE

Abstract

Under specific conditions cycloheximide treatment of Saccharomyces cerevisiae caused the accumulation of a type of polyribosome called "halfmer." Limited ribonuclease digestion of halfmers released particles from the polyribosomes identified as 40S ribosomal subunits. The data demonstrated that halfmers are polyribosomes containing an additional 40S ribosomal subunit attached to the messenger ribonucleic acid. Protein gel electrophoretic analysis of halfmers revealed numerous nonribosomal proteins. Two of these proteins comigrate with subunits of yeast initiation factor eIF2.

MeSH Terms
Cycloheximide/pharmacology Eukaryotic Initiation Factor-2 Fungal Proteins/isolation & purification Molecular Conformation Peptide Initiation Factors/isolation & purification Polyribosomes/drug effects,metabolism Proteins/isolation & purification Ribosomes/drug effects,metabolism Saccharomyces cerevisiae/drug effects,metabolism
Chemicals
Eukaryotic Initiation Factor-2 Fungal Proteins Peptide Initiation Factors Proteins Cycloheximide
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Helser T L
Division of Biology and Medicine, Brown University, Providence, Rhode Island 02912.
Baan R A
Dahlberg A E
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.1.1.51-57.1981
Published
1981-01-00
Pages
51-7
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369326
Subset
IM
Grants
NIGMS NIH HHS · 5K04GM00044 · United States
NIGMS NIH HHS · GM19756 · United States
External Links
PubMed source DOI: 10.1128/mcb.1.1.51-57.1981 PMC Full Text View journal details
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