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PMID: 6767729 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Replication fork rate and origin activation during the S phase of Saccharomyces cerevisiae.

The Journal of cell biology ·Vol. 85 ·No. 1 ·1980-04-00 ·Pages 108-15

Rivin CJ, Fangman WL

Abstract

When the growth rate of the yeast Saccharomyces cerevisiae is limited with various nitrogen sources, the duration of the S phase is proportional to cell cycle length over a fourfold range of growth rates (C.J. Rivin and W. L. Fangman, 1980, J. Cell Biol. 85:96-107). Molecular parameters of the S phases of these cells were examined by DNA fiber autoradiography. Changes in replication fork rate account completely for the changes in S-phase duration. No changes in origin-to-origin distances were detected. In addition, it was found that while most adjacent replication origins are activated within a few minutes of each other, new activations occur throughout the S phase.

MeSH Terms
Ammonia/metabolism Cell Cycle DNA Replication DNA, Fungal/biosynthesis Glutamine/metabolism Interphase Kinetics Proline/metabolism Replicon Saccharomyces cerevisiae/cytology,metabolism Threonine/metabolism
Chemicals
DNA, Fungal Glutamine Threonine Ammonia Proline
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rivin C J
Fangman W L
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25 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.85.1.108
Published
1980-04-00
Pages
108-15
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2110590
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.85.1.108 PMC Full Text View journal details
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