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PMID: 6769818 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Mitogenic effects of purified outer membrane proteins from Pseudomonas aeruginosa.

Infection and immunity ·Vol. 28 ·No. 1 ·1980-04-00 ·Pages 178-84

Chen YH, Hancock RE, Mishell RI

Abstract

Three major outer membrane proteins from Pseudomonas aeruginosa PAO1 were purified and tested for their ability to stimulate resting murine lymphocytes to proliferate. It was demonstrated that picomole amounts of all three proteins were mitogenic for both intact and T-lymphocyte-depleted populations of spleen cells from C3H/HeJ mice. In contrast, they had no activity against either mature or immature thymocytes. Since the strain of mice used is unable to respond to lipopolysaccharide, we condlude that the three proteins are B-cell mitogens.

MeSH Terms

Animals B-Lymphocytes/cytology,immunology Bacterial Proteins/isolation & purification,pharmacology Cell Division Lymphocyte Activation Membrane Proteins/isolation & purification,pharmacology Mice Mitogens Pseudomonas aeruginosa/analysis T-Lymphocytes/immunology

Chemicals

Bacterial Proteins Membrane Proteins Mitogens

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Chen Y H

Hancock R E

Mishell R I

References (26)

26 references, click to expand

A new method for the extraction of R lipopolysaccharides.
Eur J Biochem. 1969 Jun;9(2):245-9 PMID: 5804498
Brain-associated theta antigen: reactivity of rabbit anti-mouse brain with mouse lymphoid cells.
Cell Immunol. 1971 Aug;2(4):353-61 PMID: 4399614
C 55 -isoprenoid alcohol phosphokinase: an extremely hydrophobic protein from the bacterial membrane.
Proc Natl Acad Sci U S A. 1971 Oct;68(10):2441-3 PMID: 5289877
PPD tuberculin--a B-cell mitogen.
Nat New Biol. 1972 Dec 13;240(102):198-200 PMID: 4539345
Processing of adenovirus 2-induced proteins.
J Virol. 1973 Aug;12(2):241-52 PMID: 4747985
Mitogenic effect of a water-soluble extract of Nocardia opaca: a comparative study with some bacterial adjuvants on spleen and peripheral lymphocytes of four mammalian species.
J Immunol. 1974 Jun;112(6):2028-35 PMID: 4207728
Genetic control of responses to bacterial lipopolysaccharides in mice. II. A gene that influences a membrane component involved in the activation of bone marrow-derived lymphocytes by lipipolysaccharides.
J Immunol. 1975 May;114(5):1462-8 PMID: 123543
The lipoprotein of the outer membrane of Escherichia coli: a B-lymphocyte mitogen.
J Exp Med. 1975 Aug 1;142(2):473-82 PMID: 1095681
Mitogenic effect of bacterial peptidoglycans possessing adjuvant activity.
J Immunol. 1975 Jul;115(1):268-71 PMID: 807626
Cellular mechanism of endotoxin unresponsiveness in C3H/HeJ mice.
J Immunol. 1976 Feb;116(2):454-61 PMID: 129492
Endotoxin protein: a B-cell mitogen and polyclonal activator of C3H/HeJ lymphocytes.
J Exp Med. 1976 Sep 1;144(3):821-7 PMID: 784892
Isolation of a lipid A bound polypeptide responsible for "LPS-initiated" mitogenesis of C3H/HeJ spleen cells.
J Exp Med. 1976 Sep 1;144(3):840-6 PMID: 182900
Induction of lymphocyte proliferation and membrane changes by lipopeptide derivatives of the lipoprotein from the outer membrane of Escherichia coli.
Z Immunitatsforsch Immunobiol. 1977 Apr;153(1):11-22 PMID: 325934
Interaction between two major outer membrane proteins of Escherichia coli: the matrix protein and the lipoprotein.
J Bacteriol. 1978 Jan;133(1):329-35 PMID: 338585
Preferential release of new outer membrane fragments by exponentially growing Escherichia coli.
Biochim Biophys Acta. 1978 Apr 4;508(2):287-95 PMID: 346062
Induction of myeloid colony-stimulating activity in murine monocyte tumor cell lines by macrophage activators and in a T-cell line by concanavalin A.
Cancer Res. 1978 May;38(5):1414-9 PMID: 305822
Mitogenic analysis of murine B-cell heterogeneity.
J Exp Med. 1978 Jul 1;148(1):136-47 PMID: 307586
Separation and characterization of the outer membrane of Pseudomonas aeruginosa.
J Biochem. 1978 Jul;84(1):179-91 PMID: 99443
Outer membranes of gram-negative bacteria. XIX. Isolation from Pseudomonas aeruginosa PAO1 and use in reconstitution and definition of the permeability barrier.
J Bacteriol. 1978 Oct;136(1):381-90 PMID: 101518
Isolation of characterization of a major outer membrane protein of Pseudomonas aeruginosa. Evidence for the occurrence of a lipoprotein.
J Biochem. 1979 Jan;85(1):115-22 PMID: 104984
Endotoxin protein is a mitogen and polyclonal activator of human B lymphocytes.
J Exp Med. 1979 Mar 1;149(3):713-23 PMID: 311814
Protein I and protein II from the outer membrane of Escherichia coli are mouse B-lymphocyte mitogens.
Z Immunitatsforsch Immunobiol. 1979 Jun;155(5):387-98 PMID: 382663
Identification of the protein producing transmembrane diffusion pores in the outer membrane of Pseudomonas aeruginosa PA01.
Biochim Biophys Acta. 1979 Jul 5;554(2):323-31 PMID: 114220
Isolation and characterization of major outer membrane proteins of Pseudomonas aeruginosa strain PAO with special reference to peptidoglycan-associated protein.
J Biochem. 1979 Oct;86(4):979-89 PMID: 115859
A novel peptidoglycan-associated lipoprotein found in the cell envelope of Pseudomonas aeruginosa and Escherichia coli.
J Biochem. 1979 Oct;86(4):991-1000 PMID: 115860
Outer membrane of Pseudomonas aeruginosa: heat- 2-mercaptoethanol-modifiable proteins.
J Bacteriol. 1979 Dec;140(3):902-10 PMID: 118160

Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.28.1.178-184.1980

Published

1980-04-00

Pages

178-84

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC550909

Subset

External Links

PubMed source DOI: 10.1128/iai.28.1.178-184.1980 PMC Full Text View journal details

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Mitogenic effects of purified outer membrane proteins from Pseudomonas aeruginosa.

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