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PMID: 6776524 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Induction of translatable mRNA for dopa decarboxylase in Drosophila: an early response to ecdysterone.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 7 ·1980-07-00 ·Pages 4175-9

Kraminsky GP, Clark WC, Estelle MA, Gietz RD, Sage BA, O'Connor JD, Hodgetts RB

Abstract

Ecdysteroid titer and dopa decarboxylase (aromatic-L-amino-acid carboxy-lyase, EC 4.1.1.28) activity were determined throughout the life cycle of Drosophila melanogaster. Five peaks in the amount of hormone were observed, which preceeded five dopa decarboxylase peaks by times ranging from 5 to 58 hr. Late in the third instar the hormone and enzyme maxima are nearly coincident. The increase in enzyme activity observed at this time is paralleled by an increase in translatable dopa decarboxylase mRNA. To obtain evidence that ecdysterone induces the appearance of this mRNA we made use of the temperature-sensitive ecd1 mutant. Garen et al. [Garen, A., Kauvar, L. & Lepesant, J.-A. (1977) Proc. Natl, Acad. Sci. USA 74, 5099-5103] have shown that when third instar mutant larvae are kept at 29 degrees C, the ecdysteroid titer remains low. In such larvae we show that the normal increase in dopa decarboxylase activity fails to appear, and no translatable dopa decarboxylase mRNA can be detected. Exogenous feeding of ecdysterone to these larvae results in a rapid synthesis of dopa decarboxylase in the epidermal cells. In addition, a parallel increase in translatable dopa decarboxylase mRNA occurs, which may be a primary response of these target cells to ecdysterone.

MeSH Terms
Animals Aromatic-L-Amino-Acid Decarboxylases/genetics Cell-Free System Dopa Decarboxylase/biosynthesis,genetics Drosophila melanogaster/growth & development Ecdysterone/pharmacology Enzyme Induction/drug effects Epidermis/metabolism Protein Biosynthesis RNA, Messenger/genetics Transcription, Genetic/drug effects
Chemicals
RNA, Messenger Ecdysterone Dopa Decarboxylase Aromatic-L-Amino-Acid Decarboxylases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Kraminsky G P
Clark W C
Estelle M A
Gietz R D
Sage B A
O'Connor J D
Hodgetts R B
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.7.4175
Published
1980-07-00
Pages
4175-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC349793
Subset
IM
Grants
NINDS NIH HHS · NS-08990 · United States
External Links
PubMed source DOI: 10.1073/pnas.77.7.4175 PMC Full Text View journal details
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