Home LiteratureArticle Details
PMID: 6778384 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Penicillin-binding proteins of penicillin-susceptible and intrinsically resistant Neisseria gonorrhoeae.

Antimicrobial agents and chemotherapy ·Vol. 18 ·No. 5 ·1980-11-00 ·Pages 730-7

Dougherty TJ, Koller AE, Tomasz A

Abstract

The penicillin-binding proteins (PBPs) of Neisseria gonorrhoeae were investigated by using [3H]benzylpenicillin of high specific activity. This made it possible to label the PBPs both in cytoplasmic membranes and in the membranes of actively growing cells (in vivo labeling). A total of 20 strains isolated from different geographic locales showed the same pattern of three major PBPs, which had molecular weights of approximately 90,000, 63,000, and 48,000. Five clinical isolates of intrinsically penicillin-resistant gonococci each exhibited reduced penicillin binding of PBPs 1 and 2. The construction of an isogenic set of transformants with increasing levels of penicillin resistance indicated that the penA gene was associated with a decrease in penicillin binding fo PBP 2. Decreased binding to PBP 1 is likely to accompany the newly reported pem and tem genes, which govern to highest level of penicillin resistance.

MeSH Terms
Bacterial Proteins/metabolism Carrier Proteins/metabolism Hexosyltransferases Muramoylpentapeptide Carboxypeptidase Neisseria gonorrhoeae/drug effects,metabolism Penicillin Resistance Penicillin-Binding Proteins Penicillins/metabolism,pharmacology Peptidyl Transferases Protein Binding Transformation, Bacterial
Chemicals
Bacterial Proteins Carrier Proteins Penicillin-Binding Proteins Penicillins Peptidyl Transferases Hexosyltransferases Muramoylpentapeptide Carboxypeptidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Dougherty T J
Koller A E
Tomasz A
References (31)
31 references, click to expand
  1. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
    J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
  2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  3. Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane.
    J Biol Chem. 1972 Jun 25;247(12):3962-72 PMID: 4555955
  4. Resolution of bacterial proteins by polyacrylamide gel electrophoresis on slabs. Membrane, soluble, and periplasmic fractions.
    J Biol Chem. 1974 Jan 25;249(2):634-44 PMID: 4129205
  5. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
    Eur J Biochem. 1974 Jul 1;46(1):83-8 PMID: 4850204
  6. The barrier function of the gram-negative envelope.
    Ann N Y Acad Sci. 1974 May 10;235(0):109-29 PMID: 4212391
  7. Cell envelope of Neisseria gonorrhoeae: outer membrane and peptidoglycan composition of penicillin-sensitive and-resistant strains.
    Infect Immun. 1975 Jun;11(6):1332-41 PMID: 806526
  8. Genetic analysis of drug resistance in Neisseria gonorrhoeae: identification and linkage relationships of loci controlling drug resistance.
    Antimicrob Agents Chemother. 1975 May;7(5):676-81 PMID: 807159
  9. Possible mechanism of decreased susceptibility of Neisseria gonorrhoeae to penicillin.
    Antimicrob Agents Chemother. 1975 Jun;7(6):788-92 PMID: 808158
  10. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
    Eur J Biochem. 1975 Aug 15;56(2):335-41 PMID: 1175627
  11. Purification to homogeneity and properties of two D-alanine carboxypeptidases I From Escherichia coli.
    J Biol Chem. 1976 Jan 25;251(2):414-23 PMID: 1391
  12. Altered penicillin-binding components in penicillin-resistant mutants of Bacillus subtilis.
    Proc Natl Acad Sci U S A. 1976 Jun;73(6):1816-20 PMID: 819927
  13. Properties of the penicillin-binding proteins of Escherichia coli K12,.
    Eur J Biochem. 1977 Jan;72(2):341-52 PMID: 319999
  14. Altered outer membrane protein in different colonial types of Neisseria gonorrhoeae.
    J Bacteriol. 1977 Mar;129(3):1623-7 PMID: 403181
  15. Hydroxylaminolysis of penicillin binding componenets is enzymatically catalyzed.
    J Biol Chem. 1977 Nov 10;252(21):7525-9 PMID: 410805
  16. Differential binding of penicillin by membrane fractions from penicillin-susceptible and -resistant gonococci.
    Antimicrob Agents Chemother. 1978 Apr;13(4):589-97 PMID: 96727
  17. Escherichia coli resistance to beta-lactam antibiotics through a decrease in the affinity of a target for lethality.
    Nature. 1978 Aug 17;274(5672):713-5 PMID: 209344
  18. Cell envelope alterations in antibiotic-sensitive and-resistant strains of Neisseria gonorrhoeae.
    J Bacteriol. 1978 Oct;136(1):391-401 PMID: 101519
  19. Outer-membrane penetration barriers as components of intrinsic resistance to beta-lactam and other antibiotics in Escherichia coli K-12.
    Antimicrob Agents Chemother. 1979 Feb;15(2):182-9 PMID: 106773
  20. Specificity of DNA uptake in genetic transformation of gonococci.
    Biochem Biophys Res Commun. 1979 Jan 15;86(1):97-104 PMID: 107953
  21. Penetrability of the outer membrane of Neisseria gonorrhoeae in relation to acquired resistance to penicillin and other antibiotics.
    Antimicrob Agents Chemother. 1979 Jun;15(6):820-7 PMID: 112915
  22. Comparative studies of penicillin-binding proteins in Pseudomonas aeruginosa and Escherichia coli.
    Eur J Biochem. 1979 Oct;100(1):41-9 PMID: 114388
  23. Competition of beta-lactam antibiotics for the penicillin-binding proteins of Pseudomonas aeruginosa, Enterobacter cloacae, Klebsiella aerogenes, Proteus rettgeri, and Escherichia coli: comparison with antibacterial activity and effects upon bacterial morphology.
    Antimicrob Agents Chemother. 1979 Sep;16(3):325-8 PMID: 116592
  24. Comparison of the penicillin-binding proteins of different strains of Neisseria gonorrhoeae.
    Antimicrob Agents Chemother. 1979 Sep;16(3):336-40 PMID: 116593
  25. Polygenes and modifier genes for tetracycline and penicillin resistance in Neisseria gonorrhoeae.
    J Gen Microbiol. 1980 Mar;117(1):103-10 PMID: 6771365
  26. Extent of peptide cross-linking in the peptidoglycan of Neisseria gonorrhoeae.
    Infect Immun. 1980 Jun;28(3):867-75 PMID: 6772568
  27. Penetration of beta-lactam antibiotics into their target enzymes in Pseudomonas aeruginosa: comparison of a highly sensitive mutant with its parent strain.
    Antimicrob Agents Chemother. 1980 Jul;18(1):94-100 PMID: 6774666
  28. Multiple changes of penicillin-binding proteins in penicillin-resistant clinical isolates of Streptococcus pneumoniae.
    Antimicrob Agents Chemother. 1980 Mar;17(3):364-71 PMID: 7425601
  29. Penicillin-binding proteins of multiply antibiotic-resistant South African strains of Streptococcus pneumoniae.
    Antimicrob Agents Chemother. 1980 Mar;17(3):434-42 PMID: 6903436
  30. Mode of action of beta-lactam antibiotics.
    Rev Infect Dis. 1979 Jan-Feb;1(1):39-54 PMID: 400939
  31. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.18.5.730
Published
1980-11-00
Pages
730-7
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC284083
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.18.5.730 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2