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PMID: 6779279 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Protein p3 is linked to the DNA of phage phi 29 through a phosphoester bond between serine and 5'-dAMP.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 11 ·1980-11-00 ·Pages 6425-8

Hermoso JM, Salas M

Abstract

To investigate the role of protein p3 in bacteriophage phi 29 initiation of replication, we have studied the nature of the covalent linkage between protein p3 and phi 29 DNA. The protein-DNA compound was digested with micrococcal nuclease and pronase resulting in a nucleotidyl-peptide that was further digested by alkaline phosphatase and snake venom phosphodiesterase yielding 5'-dAMP. The DNA-protein linkage is sensitive to alkali. Treatment of the nucleotidyl-peptide with 0.1 M NaOH at 37 degrees C for 3 hr after phosphatase digestion released 5'-dAMP. Hydrolysis of the nucleotidyl-peptide with 5.8 M HCl at 110 degrees C for 90 min yielded O-phosphoserine. These results, together with the sensitivity of the DNA-protein linkage to snake venom phosphodiesterase and its resistance to hydroxylamine, indicate that protein p3 is covalently linked to phi 29 DNA through a phosphoester bond between L-serine and 5'-dAMP, namely a O,5'-deoxyadenylyl-L-serine bond.

MeSH Terms
Adenosine Monophosphate/metabolism Bacillus subtilis Bacteriophages/genetics Carrier Proteins/metabolism DNA/metabolism DNA Replication DNA, Viral/metabolism DNA-Binding Proteins Deoxyribonucleoproteins/metabolism Serine/metabolism Viral Proteins/metabolism Virus Replication
Chemicals
Carrier Proteins DNA, Viral DNA-Binding Proteins Deoxyribonucleoproteins Viral Proteins Adenosine Monophosphate Serine DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hermoso J M
Salas M
References (30)
30 references, click to expand
  1. Viral protein synthesis in bacteriophage phi 29-infected Bacillus subtilis.
    J Virol. 1973 Nov;12(5):1149-59 PMID: 4203085
  2. Phosphorylation of ribosomal proteins in rabbit reticulocytes. A cell-free system with ribosomal protein kinase activity.
    Biochemistry. 1971 Jan 19;10(2):197-203 PMID: 4321662
  3. The structure of replicating adenovirus 2 DNA molecules.
    Cell. 1977 Dec;12(4):1007-20 PMID: 597856
  4. Ion-exchange thin-layer chromatography. XIV. Separation of nucleotide sugars and nucleoside monophosphates on PEI-cellulose.
    Anal Biochem. 1965 Dec;13(3):575-9 PMID: 5881317
  5. Presence of a covalently linked protein on calicivirus RNA.
    J Gen Virol. 1978 Nov;41(2):443-6 PMID: 569187
  6. Protein bound to the genome RNAs of cowpea mosaic virus.
    Eur J Biochem. 1978 Dec 1;92(1):45-51 PMID: 215411
  7. A micromethod for base analysis of 32P-labeled oligoribonulcleotides.
    Anal Biochem. 1977 Nov;83(1):222-7 PMID: 920941
  8. Structural proteins of bacteriophage phi 29.
    Virology. 1971 Sep;45(3):567-76 PMID: 4108183
  9. Structure of replicating DNA molecules of Bacillus subtilis bacteriophage phi 29.
    J Virol. 1980 Apr;34(1):187-99 PMID: 6768899
  10. Characterization of a protein covalently linked to the 5' termini of the DNA of Bacillus subtilis phage phi29.
    J Mol Biol. 1978 Feb 25;119(2):269-91 PMID: 416224
  11. The protein covalently linked to the 5' termini of the DNA of Bacillus subtilis phage phi 29 is involved in the initiation of DNA replication.
    Virology. 1980 Jul 15;104(1):84-96 PMID: 6771916
  12. Identification of a protein linked to the ends of adenovirus DNA.
    Cell. 1977 Jun;11(2):283-95 PMID: 196758
  13. A protein linked to the 5' termini of both RNA components of the cowpea mosaic virus genome.
    Nucleic Acids Res. 1978 Dec;5(12):4505-22 PMID: 745988
  14. Genome-linked protein associated with the 5' termini of bacteriophage phi29 DNA.
    J Virol. 1978 Sep;27(3):776-83 PMID: 100608
  15. A protein covalently linked to poliovirus genome RNA.
    Proc Natl Acad Sci U S A. 1977 Jan;74(1):59-63 PMID: 189316
  16. Bacteriophage phi29 terminal protein: its association with the 5' termini of the phi29 genome.
    J Virol. 1978 Dec;28(3):895-904 PMID: 731797
  17. Protein covalently linked to foot-and-mouth disease virus RNA.
    Nature. 1977 Aug 18;268(5621):648-50 PMID: 197422
  18. Evidence for phosphoproteins in reovirus.
    Virology. 1975 Apr;64(2):505-12 PMID: 1169848
  19. Protein is linked to the 5' end of poliovirus RNA by a phosphodiester linkage to tyrosine.
    J Biol Chem. 1978 Aug 10;253(15):5263-6 PMID: 209034
  20. Identification of the protein firmly bound to the ends of bacteriophage phi 29 DNA.
    Virology. 1978 Feb;84(2):279-92 PMID: 203093
  21. Proteins induced in Bacillus subtilis infected with bacteriophage phi 29.
    Virology. 1973 Nov;56(1):291-9 PMID: 4200881
  22. The genome-linked protein of picornaviruses. IV. Difference in the VPg's of encephalomyocarditis virus and poliovirus as evidence that the genome-linked proteins are virus-coded.
    Virology. 1978 Aug;89(1):112-8 PMID: 210567
  23. Esters of phosphoric acid: Phosphoryl hydroxyamino-acids.
    Biochem J. 1941 Apr;35(4):461-9 PMID: 16747344
  24. Evidence for blocked 5'-termini in human adenovirus DNA.
    Virology. 1977 Jan;76(1):380-94 PMID: 835234
  25. Membrane synthesis in synchronous cultures of Bacillus subtilis 168.
    J Bacteriol. 1973 Oct;116(1):397-409 PMID: 4126817
  26. Relative alkali stability of some peptide o-phosphoserine and o-phosphothreonine esters.
    FEBS Lett. 1980 Feb 11;110(2):308-12 PMID: 15851356
  27. Covalent linkage of a protein to a defined nucleotide sequence at the 5'-terminus of virion and replicative intermediate RNAs of poliovirus.
    Proc Natl Acad Sci U S A. 1977 Mar;74(3):961-5 PMID: 191841
  28. RNA polymerase binding sites and transcription map of the DNA of Bacillus subtilis phage phi29.
    J Mol Biol. 1979 Feb 5;127(4):411-36 PMID: 107317
  29. Physical map of bacteriophage phi29 DNA.
    Virology. 1976 Oct 15;74(2):314-23 PMID: 824814
  30. O4-(5'-uridylyl)tyrosine is the bond between the genome-linked protein and the RNA of poliovirus.
    Proc Natl Acad Sci U S A. 1978 Oct;75(10):4868-72 PMID: 217003
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.11.6425
Published
1980-11-00
Pages
6425-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC350297
Subset
IM
Grants
NIGMS NIH HHS · R01 GM27242-01 · United States
External Links
PubMed source DOI: 10.1073/pnas.77.11.6425 PMC Full Text View journal details
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