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PMID: 678013 Published · ppublish English Journal Article

Degradation of microbodies in relation to activities of alcohol oxidase and catalase in Candida boidinii.

Archives of microbiology ·Vol. 117 ·No. 1 ·1978-04-27 ·Pages 67-72

Bormann C, Sahm H

Abstract

Degradation of microbodies in the methanol-utilizing yeast Candida boidinii was mainly studied by electron microscopical observation. The yeast cells precultured on methanol medium contained five to six microbodies per section and showed high activities of alcohol oxidase, catalase, formaldehyde dehydrogenase and formate dehydrogenase. When the precultured cells were transferred into an ethanol medium the number of microbodies and concomitantly the activities of alcohol oxidase and catalase decreased. After 6 h of cultivation microbodies were hardly detected. Also the activity of alcohol oxidase was not measurable and catalase activity was reduced to one tenth, whereas the activities of formaldehyde dehydrogenase and formate dehydrogenase decreased only to about 70%. Experiments with methanol-grown cells transferred into an ethanol medium without nitrogen source indicated that the inactivation of alcohol oxidase and catalase does not require protein synthesis. However, the reappearance of these enzymes is presumably due to de novo protein synthesis as shown by experiments with cycloheximide.

MeSH Terms
Alcohol Oxidoreductases/metabolism Aldehyde Oxidoreductases/metabolism Candida/enzymology,metabolism,ultrastructure Catalase/metabolism Cycloheximide/pharmacology Ethanol/metabolism Methanol/metabolism Microbodies/ultrastructure
Chemicals
Ethanol Cycloheximide Alcohol Oxidoreductases Catalase Aldehyde Oxidoreductases Methanol
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bormann C
Sahm H
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23 references, click to expand
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Article Info
Journal
Archives of microbiology
Abbr.
Arch Microbiol
ISSN
0302-8933
DOI
10.1007/BF00689353
Published
1978-04-27
Pages
67-72
Language
English
Region
Germany
NLM ID
0410427
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00689353 View journal details
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