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PMID: 6780524 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Entry of double-stranded deoxyribonucleic acid during transformation of Neisseria gonorrhoeae.

Journal of bacteriology ·Vol. 145 ·No. 1 ·1981-01-00 ·Pages 638-40

Biswas GD, Sparling PF

Abstract

The state of donor deoxyribonucleic acid after entry into competent cells was examined by assaying the transformed cell lysates for donor-marker transforming activity and density of donor deoxyribonucleic acid in CsCl gradients. The experiments showed that deoxyribonucleic acid entered in native, double-stranded form.

MeSH Terms
DNA, Bacterial Neisseria gonorrhoeae/genetics Nucleic Acid Conformation Transformation, Bacterial
Chemicals
DNA, Bacterial
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Biswas G D
Sparling P F
References (10)
10 references, click to expand
  1. Loss of activity of transforming deoxyribonucleic acid after uptake by Haemophilus influenzae.
    J Bacteriol. 1965 Oct;90(4):873-83 PMID: 5294816
  2. Fate of transforming DNA in the Haemophilus influenzae transformation system.
    J Mol Biol. 1965 Sep;13(2):554-70 PMID: 5295582
  3. Genetic transformation of Neisseria gonorrhoeae to streptomycin resistance.
    J Bacteriol. 1966 Nov;92(5):1364-71 PMID: 4958881
  4. On the nature of recombinants formed during transformation in Hemophilus influenzae.
    J Gen Physiol. 1966 Jul;49(6):197-209 PMID: 5298073
  5. Sorption of DNA and RNA during transformation of Neisseria meningitidis.
    Acta Pathol Microbiol Scand B Microbiol Immunol. 1971;79(4):563-71 PMID: 5000800
  6. Fate of transforming deoxyribonucleate in Bacillus subtilis.
    J Bacteriol. 1971 Nov;108(2):680-9 PMID: 5001868
  7. Factors affecting genetic transformation of Neisseria gonorrhoeae.
    J Bacteriol. 1977 Feb;129(2):983-92 PMID: 14116
  8. Fate of transforming deoxyribonucleate following fixation by transformable bacteria.
    Nature. 1960 Sep 17;187:1002-6 PMID: 13701116
  9. NEISSERIA GONORRHOEAE. I. VIRULENCE GENETICALLY LINKED TO CLONAL VARIATION.
    J Bacteriol. 1963 Jun;85:1274-9 PMID: 14047217
  10. FATE OF TRANSFORMING DEOXYRIBONUCLEIC ACID IN BACILLUS SUBTILIS.
    J Bacteriol. 1965 May;89:1250-5 PMID: 14292994
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
Published
1981-01-00
Pages
638-40
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC217316
Subset
IM
Grants
NIAID NIH HHS · AI15036 · United States
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